caa a22 4500 605534888 CHVBK 20210128100844.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10517-015-2903-z doi (NATIONALLICENCE)springer-10.1007/s10517-015-2903-z Cuprizone Model as a Tool for Preclinical Studies of the Efficacy of Multiple Sclerosis Diagnosis and Therapy [Elektronische Daten] [T. Abakumova, A. Kuz'kina, M. Zharova, D. Pozdeeva, I. Gubskii, I. Shepeleva, O. Antonova, N. Nukolova, Z. Kekelidze, V. Chekhonin] To study demyelination and remyelination processes and their response to different drugs, a protocol for modeling multiple sclerosis using the copper chelator cuprizone was developed. Magnetic resonance imaging confirmed the presence of demyelination lesions on week 4 of 0.6% cuprizone-containing diet. Immunohistochemical staining with polyclonal antibodies to glial fibrillary acidic protein (pAb GFAP) confirmed the increase in the number of reactive astrocytes on week 4 of diet and during remyelination (week 2 after diet). Analysis of neurophysiological functions in mice with cuprizone-induced demyelination revealed motor and behavioral deficits. This model can be used as a tool for preclinical studies of the efficiency of multiple sclerosis diagnostic and therapy. Springer Science+Business Media New York, 2015 multiple sclerosis nationallicence cuprizone nationallicence demyelination nationallicence remyelination nationallicence Abakumova T. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut Kuz'kina A. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut Zharova M. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut Pozdeeva D. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut Gubskii I. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut Shepeleva I. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut Antonova O. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut Nukolova N. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut Kekelidze Z. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut Chekhonin V. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/1(2015-05-01), 111-115 0007-4888 159:1<111 2015 159 10517 https://doi.org/10.1007/s10517-015-2903-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2903-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abakumova T. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Kuz'kina A. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Zharova M. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Pozdeeva D. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Gubskii I. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Shepeleva I. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Antonova O. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Nukolova N. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Kekelidze Z. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Chekhonin V. V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/1(2015-05-01), 111-115 0007-4888 159:1<111 2015 159 10517