caa a22 4500 605534977 CHVBK 20210128100845.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10517-015-2908-7 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2908-7 Peculiarities of Gene Transfer into Mesenchymal Stem Cells [Elektronische Daten] [N. Sats, I. Shipunova, A. Bigil'diev, D. Kostyushev, N. Drize] Murine mesenchymal stem cells in long-term bone marrow culture were genetically labeled using lentiviral vector carrying enhanced green fluorescent protein (eGFP) reporter gene under SFFV promoter or without it. We studied the developmental fate of labeled mesenchymal stem cells in stromal cell layers of long-term bone marrow culture and in ectopic hemopoietic foci formed by these stromal layers under the renal capsule of syngeneic mice. The frequency of labeled polypotent stromal precursors (fibroblast CFU) was analyzed in adherent cell layers of long-term culture and ectopic foci formed from them. The proportion of labeled fibroblast CFU in ectopic foci increased by 10 times in case of implantation of adherent cell layers infected with lentivirus containing eGFP reporter gene without promoter. eGFP expression leads to rejection of labeled stromal cells. Labeling with eGFP-carrying vector without promoter makes possible long-term tracking of mesenchymal stromal cells. Springer Science+Business Media New York, 2015 stromal mesenchymal cells nationallicence fibroblast colony-forming units nationallicence lentiviral vector nationallicence focus of ectopic hemopoiesis nationallicence long-term bone marrow culture nationallicence Sats N. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut Shipunova I. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut Bigil'diev A. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut Kostyushev D. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut Drize N. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/1(2015-05-01), 134-137 0007-4888 159:1<134 2015 159 10517 https://doi.org/10.1007/s10517-015-2908-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2908-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sats N. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Shipunova I. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Bigil'diev A. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Kostyushev D. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Drize N. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/1(2015-05-01), 134-137 0007-4888 159:1<134 2015 159 10517