caa a22 4500 605535094 CHVBK 20210128100846.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10517-015-3079-2 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3079-2 Stimulation of Cardiomyocyte Regeneratory Reactions under Conditions of Cytopathic Hypercholesterolemia [Elektronische Daten] [E. Lushnikova, E. Yuzhik, M. Klinnikova, L. Nepomnyashchikh] The regeneratory reactions of cardiomyocytes in the heart under conditions of cytopathic exposure to hypercholesterolemia and during verapamil treatment were studied by immunohistochemical detection of proliferation marker Ki-67 and evaluation of cardiomyocyte count. A 30-day exposure of rats to atherogenic diet led to an increase of Ki-67+ cardiomyocytes by 14.5-16.7 times (p<0.05). The Ki-67 label index in cardiomyocytes remained higher than normally (8-9-fold; p<0.05) after 64 days. It remained elevated (8-11-fold; p<0.05) after verapamil treatment. Evaluation of cardiomyocyte count and of their nuclear status detected various regeneratory strategies: increase of the total cardiomyocyte count, with increase of the percentage of mononuclear cardiomyocytes (particularly in response to verapamil in group 1 rats); decrease of total cardiomyocyte count with increase of the percentage of multinuclear cardiomyocytes (particularly in group 2 rats in response to verapamil). Springer Science+Business Media New York, 2015 hypercholesterolemia nationallicence cardiomyocytes nationallicence regeneration nationallicence verapamil nationallicence Ki-67 nationallicence Lushnikova E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Yuzhik E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Klinnikova M. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Nepomnyashchikh L. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/6(2015-10-01), 796-800 0007-4888 159:6<796 2015 159 10517 https://doi.org/10.1007/s10517-015-3079-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3079-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lushnikova E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Yuzhik E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Klinnikova M. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Nepomnyashchikh L. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/6(2015-10-01), 796-800 0007-4888 159:6<796 2015 159 10517