Phenotypical Differences in Neuronal Cultures Derived via Reprogramming the Fibroblasts from Patients Carrying Mutations in Parkinsonian Genes LRRK2 and PARK2
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| 024 | 7 | 0 | |a 10.1007/s10517-015-3072-9 |2 doi |
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| 245 | 0 | 0 | |a Phenotypical Differences in Neuronal Cultures Derived via Reprogramming the Fibroblasts from Patients Carrying Mutations in Parkinsonian Genes LRRK2 and PARK2 |h [Elektronische Daten] |c [E. Konovalova, E. Novosadova, I. Grivennikov, S. Illarioshkin] |
| 520 | 3 | |a Fibroblasts isolated from skin biopsy specimens from patients with genetic forms of Parkinson's disease, carriers of mutations in LRRK2 and PARK2 genes, and from a healthy volunteer were reprogrammed using lentiviral vectors into induced pluripotent stem cells (iPSC). iPSC were differentiated into neuron-like cells using a cocktail of differentiation factors (N2, B27, and Noggin). The iPSC lines derived from patients with different mutations and from a healthy volunteer cultured under the same conditions were characterized by different proportion of neuronal precursors and differentiated neurons. Control Po2 line contained 56% precursors, while B15 line with LRRK2 gene mutation (G2019S) contained 35% precursor cells. Similar regularities were characteristic of Tr5 culture carrying compound heterozygous mutations in PARK2 gene (del202-203AG and IVS1+1G/A) and containing 4% neuronal precursors. Further comparative studies of iPSC carrying various mutations and comparison with normal human cells will help to understand the molecular pathogenesis of some genetic variants of Parkinson's disease. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a induced pluripotent stem cells |2 nationallicence | |
| 690 | 7 | |a fibroblasts |2 nationallicence | |
| 690 | 7 | |a neurons |2 nationallicence | |
| 690 | 7 | |a cell reprogramming |2 nationallicence | |
| 690 | 7 | |a Parkinson's disease |2 nationallicence | |
| 700 | 1 | |a Konovalova |D E. |u Research Center of Neurology, Moscow, Russia |4 aut | |
| 700 | 1 | |a Novosadova |D E. |u Institute of Molecular Genetics, the Russian Academy of Sciences, Moscow, Russia |4 aut | |
| 700 | 1 | |a Grivennikov |D I. |u Institute of Molecular Genetics, the Russian Academy of Sciences, Moscow, Russia |4 aut | |
| 700 | 1 | |a Illarioshkin |D S. |u Research Center of Neurology, Moscow, Russia |4 aut | |
| 773 | 0 | |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 159/6(2015-10-01), 772-775 |x 0007-4888 |q 159:6<772 |1 2015 |2 159 |o 10517 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10517-015-3072-9 |q text/html |z Onlinezugriff via DOI |
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| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10517-015-3072-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Konovalova |D E. |u Research Center of Neurology, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Novosadova |D E. |u Institute of Molecular Genetics, the Russian Academy of Sciences, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Grivennikov |D I. |u Institute of Molecular Genetics, the Russian Academy of Sciences, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Illarioshkin |D S. |u Research Center of Neurology, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 159/6(2015-10-01), 772-775 |x 0007-4888 |q 159:6<772 |1 2015 |2 159 |o 10517 | ||