caa a22 4500 605535256 CHVBK 20210128100846.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10517-015-3072-9 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3072-9 Phenotypical Differences in Neuronal Cultures Derived via Reprogramming the Fibroblasts from Patients Carrying Mutations in Parkinsonian Genes LRRK2 and PARK2 [Elektronische Daten] [E. Konovalova, E. Novosadova, I. Grivennikov, S. Illarioshkin] Fibroblasts isolated from skin biopsy specimens from patients with genetic forms of Parkinson's disease, carriers of mutations in LRRK2 and PARK2 genes, and from a healthy volunteer were reprogrammed using lentiviral vectors into induced pluripotent stem cells (iPSC). iPSC were differentiated into neuron-like cells using a cocktail of differentiation factors (N2, B27, and Noggin). The iPSC lines derived from patients with different mutations and from a healthy volunteer cultured under the same conditions were characterized by different proportion of neuronal precursors and differentiated neurons. Control Po2 line contained 56% precursors, while B15 line with LRRK2 gene mutation (G2019S) contained 35% precursor cells. Similar regularities were characteristic of Tr5 culture carrying compound heterozygous mutations in PARK2 gene (del202-203AG and IVS1+1G/A) and containing 4% neuronal precursors. Further comparative studies of iPSC carrying various mutations and comparison with normal human cells will help to understand the molecular pathogenesis of some genetic variants of Parkinson's disease. Springer Science+Business Media New York, 2015 induced pluripotent stem cells nationallicence fibroblasts nationallicence neurons nationallicence cell reprogramming nationallicence Parkinson's disease nationallicence Konovalova E. Research Center of Neurology, Moscow, Russia aut Novosadova E. Institute of Molecular Genetics, the Russian Academy of Sciences, Moscow, Russia aut Grivennikov I. Institute of Molecular Genetics, the Russian Academy of Sciences, Moscow, Russia aut Illarioshkin S. Research Center of Neurology, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/6(2015-10-01), 772-775 0007-4888 159:6<772 2015 159 10517 https://doi.org/10.1007/s10517-015-3072-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3072-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Konovalova E. Research Center of Neurology, Moscow, Russia aut NATIONALLICENCE 700 1- Novosadova E. Institute of Molecular Genetics, the Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Grivennikov I. Institute of Molecular Genetics, the Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Illarioshkin S. Research Center of Neurology, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/6(2015-10-01), 772-775 0007-4888 159:6<772 2015 159 10517