caa a22 4500 605535272 CHVBK 20210128100846.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10517-015-3053-z doi (NATIONALLICENCE)springer-10.1007/s10517-015-3053-z Effect of NMDA, a Specific Agonist to NMDA Receptor Complex, on Rat Hippocampus [Elektronische Daten] [V. Motin, V. Yasnetsov] Removal of Mg2+ ions from perfusion medium provoked epileptiform activity in CA1 field of surviving rat hippocampal slices manifested in generation of extra population spikes. MK-801 (100 μM), a specific non-competitive antagonist to NMDA-receptor complex, prevented this effect. NMDA (20 μM), the specific agonist to this complex, produced no significant effect on the orthodromic population spikes, but when applied at concentrations of 30 or 40 μM, it inhibited them partially (by 21-28%) or almost completely (by 98-99%), correspondingly. Thus, depending on concentration, NMDA can inhibit the synaptic transmission in Schaffer collaterals-hippocampal CA1 pyramidal neurons axis without triggering the epileptiform activity. D-AP5 (50 μM), a competitive antagonist to NMDA-receptor complex, completely prevented the inhibitory effect of NMDA (40 μM). While MK-801 (100 μM) almost completely prevented the inhibitory effect of NMDA, it did not eliminate it when applied after the agonist. Thus, MK-801 can prevent the inhibitory action of NMDA on synaptic transmission in Schaffer collaterals-hippocampal CA1 pyramidal neurons axis via blocking the channel of NMDA-receptor complex, while NMDA exerts its effect only via activation of NMDA receptors. Springer Science+Business Media New York, 2015 hippocampal CA1 field nationallicence N-methyl-D-aspartate nationallicence NMDA-receptor complex nationallicence epileptiform Activity nationallicence Motin V. P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia aut Yasnetsov V. All-Russian Research Center for Safety of Bioactive Compounds, Staraya Kupavna, Moscow Region, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/6(2015-10-01), 704-707 0007-4888 159:6<704 2015 159 10517 https://doi.org/10.1007/s10517-015-3053-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3053-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Motin V. P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Yasnetsov V. All-Russian Research Center for Safety of Bioactive Compounds, Staraya Kupavna, Moscow Region, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/6(2015-10-01), 704-707 0007-4888 159:6<704 2015 159 10517