caa a22 4500 605535337 CHVBK 20210128100847.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10517-015-3070-y doi (NATIONALLICENCE)springer-10.1007/s10517-015-3070-y Stable Lentiviral Vector Transfer into Mesenchymal Stem Cells In Vivo [Elektronische Daten] [N. Sats, I. Shipunova, A. Bigil'diev, N. Drize] Green fluorescent protein (eGFP) gene was transferred into mouse mesenchymal stem cells in vivo using a lentiviral vector. In 2 months after injection of the lentivirus into the cavity of the femoral bone, up to 30% fibroblast CFU in the bone marrow of infected mice contained the alien gene. The transferred gene was found in more than 50% of adherent layers of longterm bone marrow cultures formed by mesenchymal stem cells from the infected mice bone marrow; 4% fibroblast CFU obtained from these layers were labeled. Ectopic hemopoiesis foci developed after transplantation of the bone marrow from infected mice under the renal capsule of syngeneic recipients contained bone tissue labeled with the alien gene in 57% cases and labeled fibroblast CFU in 11%. The data confirm the possibility of gene transfer with the lentiviral vectors into the mesenchymal stem cells in vivo. Springer Science+Business Media New York, 2015 stromal mesenchymal cells nationallicence fibroblast colony-forming units nationallicence lentiviral vector nationallicence focus of ectopic hemopoiesis nationallicence long-term bone marrow culture nationallicence Sats N. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut Shipunova I. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut Bigil'diev A. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut Drize N. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/6(2015-10-01), 764-767 0007-4888 159:6<764 2015 159 10517 https://doi.org/10.1007/s10517-015-3070-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3070-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sats N. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Shipunova I. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Bigil'diev A. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Drize N. Hematological Research Center, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/6(2015-10-01), 764-767 0007-4888 159:6<764 2015 159 10517