caa a22 4500 60553540X CHVBK 20210128100847.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10517-015-2974-x doi (NATIONALLICENCE)springer-10.1007/s10517-015-2974-x Morphological Evaluation of Oxidative Phosphorylation System in Myocardial Infarction under Conditions of Modified Vascular Endothelial Growth Factor Concentration [Elektronische Daten] [M. Shurygin, I. Shurygina, O. Kanya, N. Dremina, E. Lushnikova, R. Nepomnyashchikh] The relationship between vascular endothelium growth factor (VEGF) and cardiomyocyte oxidative phosphorylation level in experimental myocardial infarction, caused by diathermocoagulation of the periconical ventricular artery, was studied by immunofluorescent microscopy. Staining showed uneven distribution of cytochrome c in the mitochondria in the focus of myocardial infarction 2 h after the operation. After 24 h uneven staining of cardiomyocytes was found in the peri-infarction zone and no staining in the zone of myocardial infarction. This indicated a significant decrease in the level of redox enzymes. This picture persisted till day 14. Intraventricular injection of VEGF to animals led to a significant increase of the immunohistochemical reaction intensity, which reached the peak by day 7. The distribution of immunohistochemical reaction products under conditions of VEGF blocking was about the same as in spontaneous postinfarction reparative restitution. Our data indicated that increase of VEGF concentration in the myocardium maintained and stimulated oxidative phosphorylation in cardiomyocytes during the postinfarction period. Springer Science+Business Media New York, 2015 experimental myocardial infarction nationallicence vascular endothelial growth factor (VEGF) nationallicence oxidative phosphorylation nationallicence immunofluorescent microscopy nationallicence Shurygin M. Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia aut Shurygina I. Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia aut Kanya O. Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia aut Dremina N. Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia aut Lushnikova E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Nepomnyashchikh R. Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/3(2015-07-01), 402-405 0007-4888 159:3<402 2015 159 10517 https://doi.org/10.1007/s10517-015-2974-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2974-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shurygin M. Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia aut NATIONALLICENCE 700 1- Shurygina I. Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia aut NATIONALLICENCE 700 1- Kanya O. Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia aut NATIONALLICENCE 700 1- Dremina N. Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia aut NATIONALLICENCE 700 1- Lushnikova E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Nepomnyashchikh R. Irkutsk Scientific Center of Surgery and Traumatology, Irkutsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/3(2015-07-01), 402-405 0007-4888 159:3<402 2015 159 10517