Estimation of Functional Activity of Ionized Calcium for Complement System Reactivity Assessment
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| 024 | 7 | 0 | |a 10.1007/s10517-015-2948-z |2 doi |
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| 245 | 0 | 0 | |a Estimation of Functional Activity of Ionized Calcium for Complement System Reactivity Assessment |h [Elektronische Daten] |c [B. Shoibonov, D. Grigor'eva, A. Shabalina, M. Kostyreva, V. Baronets, T. Zamolodchikova] |
| 520 | 3 | |a We proposed a new indicator for evaluation of functional activity of Ca2+ in human blood serum based on lysis of sheep erythrocytes with 10% human blood serum in the presence of 0.55 mM ethylene glycol tetraacetic acid at 37оC for 10 min. After incubation, the degree of sheep erythrocytes lysis inhibition is estimated: inhibition of complement hemolytic activity <30% is considered as high functional Са2+ activity, inhibition by 31-70% corresponds to normal activity, and >71% indicates low activity. The comparative studies of complement activating function of heterophilic antibodies, complement system reactivity in the presence of 0.29 M NaCl, and functional activity of ionized Ca2+ make possible estimation of the individual's immune status. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a complement system |2 nationallicence | |
| 690 | 7 | |a ethylene glycol tetraacetic acid |2 nationallicence | |
| 690 | 7 | |a functional activity of ionized Ca2+ |2 nationallicence | |
| 690 | 7 | |a complement inhibition |2 nationallicence | |
| 700 | 1 | |a Shoibonov |D B. |u P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | |
| 700 | 1 | |a Grigor'eva |D D. |u P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | |
| 700 | 1 | |a Shabalina |D A. |u Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | |
| 700 | 1 | |a Kostyreva |D M. |u Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | |
| 700 | 1 | |a Baronets |D V. |u P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | |
| 700 | 1 | |a Zamolodchikova |D T. |u P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | |
| 773 | 0 | |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 159/3(2015-07-01), 305-308 |x 0007-4888 |q 159:3<305 |1 2015 |2 159 |o 10517 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10517-015-2948-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10517-015-2948-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shoibonov |D B. |u P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Grigor'eva |D D. |u P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shabalina |D A. |u Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kostyreva |D M. |u Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Baronets |D V. |u P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zamolodchikova |D T. |u P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 159/3(2015-07-01), 305-308 |x 0007-4888 |q 159:3<305 |1 2015 |2 159 |o 10517 | ||