caa a22 4500 605535477 CHVBK 20210128100847.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10517-015-2948-z doi (NATIONALLICENCE)springer-10.1007/s10517-015-2948-z Estimation of Functional Activity of Ionized Calcium for Complement System Reactivity Assessment [Elektronische Daten] [B. Shoibonov, D. Grigor'eva, A. Shabalina, M. Kostyreva, V. Baronets, T. Zamolodchikova] We proposed a new indicator for evaluation of functional activity of Ca2+ in human blood serum based on lysis of sheep erythrocytes with 10% human blood serum in the presence of 0.55 mM ethylene glycol tetraacetic acid at 37оC for 10 min. After incubation, the degree of sheep erythrocytes lysis inhibition is estimated: inhibition of complement hemolytic activity <30% is considered as high functional Са2+ activity, inhibition by 31-70% corresponds to normal activity, and >71% indicates low activity. The comparative studies of complement activating function of heterophilic antibodies, complement system reactivity in the presence of 0.29 M NaCl, and functional activity of ionized Ca2+ make possible estimation of the individual's immune status. Springer Science+Business Media New York, 2015 complement system nationallicence ethylene glycol tetraacetic acid nationallicence functional activity of ionized Ca2+ nationallicence complement inhibition nationallicence Shoibonov B. P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia aut Grigor'eva D. P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia aut Shabalina A. Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russia aut Kostyreva M. Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russia aut Baronets V. P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia aut Zamolodchikova T. P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/3(2015-07-01), 305-308 0007-4888 159:3<305 2015 159 10517 https://doi.org/10.1007/s10517-015-2948-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2948-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shoibonov B. P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Grigor'eva D. P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Shabalina A. Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Kostyreva M. Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Baronets V. P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Zamolodchikova T. P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/3(2015-07-01), 305-308 0007-4888 159:3<305 2015 159 10517