caa a22 4500 605535558 CHVBK 20210128100848.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10517-015-2959-9 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2959-9 Effect of Erythropoietin on Lymphocytes Apoptosis in Experimental Chronic Renal Failure [Elektronische Daten] [M. Osikov, L. Telesheva, Yu. Ageev] Recombinant human erythropoietin was injected intraperitoneally in a total dose of 900 U/kg to rats with experimental chronic renal failure. Suspension of lymphocytes from animals with chronic renal failure was used in vitro, erythropoietin was used in concentrations of 30, 15, 7.5, 3.75, and 1.88 U/liter. Intact cells (Annexin-5-FITC-/7-AAD-), cells with early signs of apoptosis (Annexin-5-FITC+/7-AAD-), cells with late signs of apoptosis and partially necrotic cells (Annexin-5-FITC+/7-AAD+), as well as cells with early signs of necrosis (Annexin-5-FITC-/7-AAD+) were differentiated by fl ow cytometry. It was found that the number of peripheral blood lymphocytes with early and late signs of apoptosis and necrosis increased in chronic renal failure. Erythropoietin at a total dose of 900 U/kg reduced the number of blood lymphocytes with signs of apoptosis and necrosis and thus elevated the number of intact lymphocytes. Erythropoietin in concentrations ranging from 1.88 to 30.0 U/liter dosedependently lowered the number of lymphocytes with early signs of apoptosis and the number of lymphocytes with the signs of late apoptosis and necrosis in vitro. Springer Science+Business Media New York, 2015 erythropoietin nationallicence lymphocytes nationallicence apoptosis nationallicence chronic renal failure nationallicence Osikov M. South Ural State Medical University, Ministry of Health of the Russian Federation, Chelyabinsk, Russia aut Telesheva L. South Ural State Medical University, Ministry of Health of the Russian Federation, Chelyabinsk, Russia aut Ageev Yu South Ural State Medical University, Ministry of Health of the Russian Federation, Chelyabinsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/3(2015-07-01), 348-350 0007-4888 159:3<348 2015 159 10517 https://doi.org/10.1007/s10517-015-2959-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2959-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Osikov M. South Ural State Medical University, Ministry of Health of the Russian Federation, Chelyabinsk, Russia aut NATIONALLICENCE 700 1- Telesheva L. South Ural State Medical University, Ministry of Health of the Russian Federation, Chelyabinsk, Russia aut NATIONALLICENCE 700 1- Ageev Yu South Ural State Medical University, Ministry of Health of the Russian Federation, Chelyabinsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/3(2015-07-01), 348-350 0007-4888 159:3<348 2015 159 10517