Disorders in the Morphology and Nanostructure of Erythrocyte Membranes after Long-term Storage of Erythrocyte Suspension: Atomic Force Microscopy Study

Verfasser / Beitragende:
[V. Moroz, A. Chernysh, E. Kozlova, V. Sergunova, O. Gudkova, S. Khoroshilov, A. Onufrievich, A. Kostin]
Ort, Verlag, Jahr:
2015
Enthalten in:
Bulletin of Experimental Biology and Medicine, 159/3(2015-07-01), 406-410
Format:
Artikel (online)
ID: 605535639
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024 7 0 |a 10.1007/s10517-015-2975-9  |2 doi 
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245 0 0 |a Disorders in the Morphology and Nanostructure of Erythrocyte Membranes after Long-term Storage of Erythrocyte Suspension: Atomic Force Microscopy Study  |h [Elektronische Daten]  |c [V. Moroz, A. Chernysh, E. Kozlova, V. Sergunova, O. Gudkova, S. Khoroshilov, A. Onufrievich, A. Kostin] 
520 3 |a Disorders in the erythrocyte morphology and structure of their membranes during long-term storage of erythrocyte suspension (30 days at 4°C) were studied by atomic force microscopy. The morphology and nanostructure of erythrocyte membranes, biochemical parameters, ion exchange parameters, and hemoglobin spectra were recorded. The transformation of erythrocyte morphology and destruction of their membranes were observed throughout the storage period. Irreversible forms of spheroechinocytes and their fragments formed by the end of storage. The concentrations of potassium ions and lactate in solution of the blood preservatiive increased, while pH value decreased. Hemolysis detected by the erythrocyte "leakage” effect was observed starting from days 16-23 of storage. 
540 |a Springer Science+Business Media New York, 2015 
690 7 |a erythrocyte suspension  |2 nationallicence 
690 7 |a storage  |2 nationallicence 
690 7 |a erythrocytes  |2 nationallicence 
690 7 |a lesions  |2 nationallicence 
700 1 |a Moroz  |D V.  |u V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia  |4 aut 
700 1 |a Chernysh  |D A.  |u V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia  |4 aut 
700 1 |a Kozlova  |D E.  |u I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Sergunova  |D V.  |u V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia  |4 aut 
700 1 |a Gudkova  |D O.  |u V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia  |4 aut 
700 1 |a Khoroshilov  |D S.  |u N. N. Burdenko Head Military Clinical Hospital, Ministry of Defense of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Onufrievich  |D A.  |u N. N. Burdenko Head Military Clinical Hospital, Ministry of Defense of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Kostin  |D A.  |u S. P. Botkin Municipal Clinical Hospital, Department of Health of Moscow, Moscow, Russia  |4 aut 
773 0 |t Bulletin of Experimental Biology and Medicine  |d Springer US; http://www.springer-ny.com  |g 159/3(2015-07-01), 406-410  |x 0007-4888  |q 159:3<406  |1 2015  |2 159  |o 10517 
856 4 0 |u https://doi.org/10.1007/s10517-015-2975-9  |q text/html  |z Onlinezugriff via DOI 
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908 |D 1  |a research-article  |2 jats 
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950 |B NATIONALLICENCE  |P 700  |E 1-  |a Moroz  |D V.  |u V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Chernysh  |D A.  |u V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Kozlova  |D E.  |u I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Sergunova  |D V.  |u V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Gudkova  |D O.  |u V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Khoroshilov  |D S.  |u N. N. Burdenko Head Military Clinical Hospital, Ministry of Defense of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Onufrievich  |D A.  |u N. N. Burdenko Head Military Clinical Hospital, Ministry of Defense of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Kostin  |D A.  |u S. P. Botkin Municipal Clinical Hospital, Department of Health of Moscow, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Bulletin of Experimental Biology and Medicine  |d Springer US; http://www.springer-ny.com  |g 159/3(2015-07-01), 406-410  |x 0007-4888  |q 159:3<406  |1 2015  |2 159  |o 10517