caa a22 4500 605535639 CHVBK 20210128100848.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10517-015-2975-9 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2975-9 Disorders in the Morphology and Nanostructure of Erythrocyte Membranes after Long-term Storage of Erythrocyte Suspension: Atomic Force Microscopy Study [Elektronische Daten] [V. Moroz, A. Chernysh, E. Kozlova, V. Sergunova, O. Gudkova, S. Khoroshilov, A. Onufrievich, A. Kostin] Disorders in the erythrocyte morphology and structure of their membranes during long-term storage of erythrocyte suspension (30 days at 4°C) were studied by atomic force microscopy. The morphology and nanostructure of erythrocyte membranes, biochemical parameters, ion exchange parameters, and hemoglobin spectra were recorded. The transformation of erythrocyte morphology and destruction of their membranes were observed throughout the storage period. Irreversible forms of spheroechinocytes and their fragments formed by the end of storage. The concentrations of potassium ions and lactate in solution of the blood preservatiive increased, while pH value decreased. Hemolysis detected by the erythrocyte "leakage” effect was observed starting from days 16-23 of storage. Springer Science+Business Media New York, 2015 erythrocyte suspension nationallicence storage nationallicence erythrocytes nationallicence lesions nationallicence Moroz V. V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia aut Chernysh A. V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia aut Kozlova E. I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia aut Sergunova V. V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia aut Gudkova O. V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia aut Khoroshilov S. N. N. Burdenko Head Military Clinical Hospital, Ministry of Defense of the Russian Federation, Moscow, Russia aut Onufrievich A. N. N. Burdenko Head Military Clinical Hospital, Ministry of Defense of the Russian Federation, Moscow, Russia aut Kostin A. S. P. Botkin Municipal Clinical Hospital, Department of Health of Moscow, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/3(2015-07-01), 406-410 0007-4888 159:3<406 2015 159 10517 https://doi.org/10.1007/s10517-015-2975-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2975-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moroz V. V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia aut NATIONALLICENCE 700 1- Chernysh A. V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia aut NATIONALLICENCE 700 1- Kozlova E. I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Sergunova V. V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia aut NATIONALLICENCE 700 1- Gudkova O. V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia aut NATIONALLICENCE 700 1- Khoroshilov S. N. N. Burdenko Head Military Clinical Hospital, Ministry of Defense of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Onufrievich A. N. N. Burdenko Head Military Clinical Hospital, Ministry of Defense of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Kostin A. S. P. Botkin Municipal Clinical Hospital, Department of Health of Moscow, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/3(2015-07-01), 406-410 0007-4888 159:3<406 2015 159 10517