Analysis of the Effect of β-Endorphin-Like Peptide Pentarphin on Intracellular Ca2+ Concentration in Early Mouse Embryos Using Fluorescent Probes

Verfasser / Beitragende:
[A. Chernov, L. Sergievich, N. Karnaukhova]
Ort, Verlag, Jahr:
2015
Enthalten in:
Bulletin of Experimental Biology and Medicine, 159/4(2015-08-01), 459-462
Format:
Artikel (online)
ID: 605535833
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024 7 0 |a 10.1007/s10517-015-2991-9  |2 doi 
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245 0 0 |a Analysis of the Effect of β-Endorphin-Like Peptide Pentarphin on Intracellular Ca2+ Concentration in Early Mouse Embryos Using Fluorescent Probes  |h [Elektronische Daten]  |c [A. Chernov, L. Sergievich, N. Karnaukhova] 
520 3 |a At the early stages of mammalian embryo development, Ca2+ influences the processes of cleavage and differentiation. An important role in these processes is played by peptide growth factors. One of them, pentarphin, is a peptide growth factor similar to the central part of β-endorphin molecule and a fragment 369-373 of IgG heavy chain. We used fluorescent Ca2+ indicators Fluo-3 AM and Fura Red AM. Pentarphin added to embryos in a concentration of 0.1 μM 1.5-2 fold increased intercellular Ca2+ level in comparison with the initial level. 
540 |a Springer Science+Business Media New York, 2015 
690 7 |a fluorescent probes  |2 nationallicence 
690 7 |a pentarphin  |2 nationallicence 
690 7 |a preimplantation embryos  |2 nationallicence 
690 7 |a intercellular Ca 2+  |2 nationallicence 
700 1 |a Chernov  |D A.  |u Institute of Theoretical and Experimental Biophysics, Moscow, Russia  |4 aut 
700 1 |a Sergievich  |D L.  |u Institute of Cell Biophysics, Pushchino, Moscow Region, Russia  |4 aut 
700 1 |a Karnaukhova  |D N.  |u Institute of Cell Biophysics, Pushchino, Moscow Region, Russia  |4 aut 
773 0 |t Bulletin of Experimental Biology and Medicine  |d Springer US; http://www.springer-ny.com  |g 159/4(2015-08-01), 459-462  |x 0007-4888  |q 159:4<459  |1 2015  |2 159  |o 10517 
856 4 0 |u https://doi.org/10.1007/s10517-015-2991-9  |q text/html  |z Onlinezugriff via DOI 
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900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
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950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s10517-015-2991-9  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Chernov  |D A.  |u Institute of Theoretical and Experimental Biophysics, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Sergievich  |D L.  |u Institute of Cell Biophysics, Pushchino, Moscow Region, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Karnaukhova  |D N.  |u Institute of Cell Biophysics, Pushchino, Moscow Region, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Bulletin of Experimental Biology and Medicine  |d Springer US; http://www.springer-ny.com  |g 159/4(2015-08-01), 459-462  |x 0007-4888  |q 159:4<459  |1 2015  |2 159  |o 10517