caa a22 4500 605535833 CHVBK 20210128100849.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10517-015-2991-9 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2991-9 Analysis of the Effect of β-Endorphin-Like Peptide Pentarphin on Intracellular Ca2+ Concentration in Early Mouse Embryos Using Fluorescent Probes [Elektronische Daten] [A. Chernov, L. Sergievich, N. Karnaukhova] At the early stages of mammalian embryo development, Ca2+ influences the processes of cleavage and differentiation. An important role in these processes is played by peptide growth factors. One of them, pentarphin, is a peptide growth factor similar to the central part of β-endorphin molecule and a fragment 369-373 of IgG heavy chain. We used fluorescent Ca2+ indicators Fluo-3 AM and Fura Red AM. Pentarphin added to embryos in a concentration of 0.1 μM 1.5-2 fold increased intercellular Ca2+ level in comparison with the initial level. Springer Science+Business Media New York, 2015 fluorescent probes nationallicence pentarphin nationallicence preimplantation embryos nationallicence intercellular Ca 2+ nationallicence Chernov A. Institute of Theoretical and Experimental Biophysics, Moscow, Russia aut Sergievich L. Institute of Cell Biophysics, Pushchino, Moscow Region, Russia aut Karnaukhova N. Institute of Cell Biophysics, Pushchino, Moscow Region, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/4(2015-08-01), 459-462 0007-4888 159:4<459 2015 159 10517 https://doi.org/10.1007/s10517-015-2991-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2991-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chernov A. Institute of Theoretical and Experimental Biophysics, Moscow, Russia aut NATIONALLICENCE 700 1- Sergievich L. Institute of Cell Biophysics, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 700 1- Karnaukhova N. Institute of Cell Biophysics, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/4(2015-08-01), 459-462 0007-4888 159:4<459 2015 159 10517