caa a22 4500 605535841 CHVBK 20210128100849.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10517-015-3004-8 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3004-8 Immunohistochemical Analysis of MMP-2 Expression in the Myocardium During the Postinfarction Period [Elektronische Daten] [L. Nepomnyashchikh, E. Lushnikova, M. Bakarev, D. Nikityuk, E. Yuzhik, M. Mzhelskaya, R. Nepomnyashchikh, M. Klinnikova, A. Karpova] Immunohistochemical analysis revealed 2.5-fold increased of expression MMP-2 in myocardium samples during the early period (up to 3 days) of postinfarction reparative regeneration. During this period, MMP-2 was detected mainly in monocytes/macrophages circulating in the blood and migrating to the necrotic zone, while expression in the intermuscular and perivascular connective tissue was lower. At later terms, with development of large focal and diffuse cardiosclerosis, MMP-2 expression significantly decreased (to the initial level) and was detected mainly in the foci of intermuscular and perivascular fibrosis, its area in the sections increased by 1.8 times. Evaluation of MMP-2 expression in the blood vessels showed that the immunohistochemical reaction was the most pronounced in the walls of new sinusoidal vessels and the minimum in the intramural arteries of medium diameter. These results attest to an important role of MMP in connective tissue remodeling (proteolytic degradation) during the early period of postinfarction reparative regeneration. The decrease in MMP-2 expression observed at later terms correlated with myocardial fibrosis progression. Springer Science+Business Media New York, 2015 myocardial infarction nationallicence cardiofi brosis nationallicence matrix metalloproteinase-2 nationallicence immunohistochemistry nationallicence Nepomnyashchikh L. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Lushnikova E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Bakarev M. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Nikityuk D. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Yuzhik E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Mzhelskaya M. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Nepomnyashchikh R. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Klinnikova M. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Karpova A. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/4(2015-08-01), 505-510 0007-4888 159:4<505 2015 159 10517 https://doi.org/10.1007/s10517-015-3004-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3004-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nepomnyashchikh L. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Lushnikova E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Bakarev M. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Nikityuk D. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Yuzhik E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Mzhelskaya M. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Nepomnyashchikh R. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Klinnikova M. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Karpova A. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/4(2015-08-01), 505-510 0007-4888 159:4<505 2015 159 10517