Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering
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| 024 | 7 | 0 | |a 10.1007/s10517-015-3014-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10517-015-3014-6 | ||
| 245 | 0 | 0 | |a Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering |h [Elektronische Daten] |c [O. Romanova, T. Grigor'ev, M. Goncharov, S. Rudyak, E. Solov'yova, S. Krasheninnikov, V. Saprykin, E. Sytina, S. Chvalun, M. Pal'tsev, A. Panteleev] |
| 520 | 3 | |a We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers. The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes). In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix. However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds. The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment. Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective. We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a keratinocytes |2 nationallicence | |
| 690 | 7 | |a fibroblasts |2 nationallicence | |
| 690 | 7 | |a biocompatibility |2 nationallicence | |
| 690 | 7 | |a skin equivalent |2 nationallicence | |
| 700 | 1 | |a Romanova |D O. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | |
| 700 | 1 | |a Grigor'ev |D T. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | |
| 700 | 1 | |a Goncharov |D M. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | |
| 700 | 1 | |a Rudyak |D S. |u MiraksBioFarma, Khimki, Moscow Region, Russia |4 aut | |
| 700 | 1 | |a Solov'yova |D E. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | |
| 700 | 1 | |a Krasheninnikov |D S. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | |
| 700 | 1 | |a Saprykin |D V. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | |
| 700 | 1 | |a Sytina |D E. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | |
| 700 | 1 | |a Chvalun |D S. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | |
| 700 | 1 | |a Pal'tsev |D M. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | |
| 700 | 1 | |a Panteleev |D A. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | |
| 773 | 0 | |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 159/4(2015-08-01), 557-566 |x 0007-4888 |q 159:4<557 |1 2015 |2 159 |o 10517 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10517-015-3014-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10517-015-3014-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Romanova |D O. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Grigor'ev |D T. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Goncharov |D M. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rudyak |D S. |u MiraksBioFarma, Khimki, Moscow Region, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Solov'yova |D E. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Krasheninnikov |D S. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Saprykin |D V. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sytina |D E. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chvalun |D S. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pal'tsev |D M. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Panteleev |D A. |u National Research Centre Kurchatov Institute, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 159/4(2015-08-01), 557-566 |x 0007-4888 |q 159:4<557 |1 2015 |2 159 |o 10517 | ||