caa a22 4500 605535868 CHVBK 20210128100849.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10517-015-3014-6 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3014-6 Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering [Elektronische Daten] [O. Romanova, T. Grigor'ev, M. Goncharov, S. Rudyak, E. Solov'yova, S. Krasheninnikov, V. Saprykin, E. Sytina, S. Chvalun, M. Pal'tsev, A. Panteleev] We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers. The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes). In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix. However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds. The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment. Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective. We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds. Springer Science+Business Media New York, 2015 keratinocytes nationallicence fibroblasts nationallicence biocompatibility nationallicence skin equivalent nationallicence Romanova O. National Research Centre Kurchatov Institute, Moscow, Russia aut Grigor'ev T. National Research Centre Kurchatov Institute, Moscow, Russia aut Goncharov M. National Research Centre Kurchatov Institute, Moscow, Russia aut Rudyak S. MiraksBioFarma, Khimki, Moscow Region, Russia aut Solov'yova E. National Research Centre Kurchatov Institute, Moscow, Russia aut Krasheninnikov S. National Research Centre Kurchatov Institute, Moscow, Russia aut Saprykin V. National Research Centre Kurchatov Institute, Moscow, Russia aut Sytina E. National Research Centre Kurchatov Institute, Moscow, Russia aut Chvalun S. National Research Centre Kurchatov Institute, Moscow, Russia aut Pal'tsev M. National Research Centre Kurchatov Institute, Moscow, Russia aut Panteleev A. National Research Centre Kurchatov Institute, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/4(2015-08-01), 557-566 0007-4888 159:4<557 2015 159 10517 https://doi.org/10.1007/s10517-015-3014-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3014-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Romanova O. National Research Centre Kurchatov Institute, Moscow, Russia aut NATIONALLICENCE 700 1- Grigor'ev T. National Research Centre Kurchatov Institute, Moscow, Russia aut NATIONALLICENCE 700 1- Goncharov M. National Research Centre Kurchatov Institute, Moscow, Russia aut NATIONALLICENCE 700 1- Rudyak S. MiraksBioFarma, Khimki, Moscow Region, Russia aut NATIONALLICENCE 700 1- Solov'yova E. National Research Centre Kurchatov Institute, Moscow, Russia aut NATIONALLICENCE 700 1- Krasheninnikov S. National Research Centre Kurchatov Institute, Moscow, Russia aut NATIONALLICENCE 700 1- Saprykin V. National Research Centre Kurchatov Institute, Moscow, Russia aut NATIONALLICENCE 700 1- Sytina E. National Research Centre Kurchatov Institute, Moscow, Russia aut NATIONALLICENCE 700 1- Chvalun S. National Research Centre Kurchatov Institute, Moscow, Russia aut NATIONALLICENCE 700 1- Pal'tsev M. National Research Centre Kurchatov Institute, Moscow, Russia aut NATIONALLICENCE 700 1- Panteleev A. National Research Centre Kurchatov Institute, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/4(2015-08-01), 557-566 0007-4888 159:4<557 2015 159 10517