caa a22 4500 605535957 CHVBK 20210128100850.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10517-015-3016-4 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3016-4 Delayed Consequences of Changes in DNA Synthesis in Cells Exposed to Single Irradiation at the End of S Phase of the Mitotic Cycle [Elektronische Daten] [I. Shabalkin, E. Grigor'eva, M. Gudkova, Yu. Stukalov] Kinetics of DNA synthesis throughout the mitotic cycle in mouse corneal epithelial cells after single γ-irradiation of cells (4 Gy) at the end of S phase was studied by the method of radioautography. It was found that single irradiation increased the duration of S phase due to reparation of damage in the cell at the expense of time that normally falls on g1 phase. During reparation, two parallel DNA synthesis processes occur in the damaged cells: de novo synthesis at the site of injury after excision of the damaged fragments (reparative synthesis) and supplementary synthesis during the repair period in the remaining undamaged genome competent for replication. During supplementary synthesis, repeats appear in DNA structure, which increases the amount of genetic material in the cell and affect S phase duration. All reparative processes take place in the cell population consisting of subpopulations of "differentiated”, "resting”, and "proliferating” cells. The changes in the proportions between the subpopulations under the influence of extreme factors can induce the appearance of metastatic cells in the population. Springer Science+Business Media New York, 2015 cell nationallicence DNA synthesis nationallicence mitotic cycle nationallicence irradiation nationallicence reparation nationallicence Shabalkin I. N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut Grigor'eva E. N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut Gudkova M. N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut Stukalov Yu N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/4(2015-08-01), 572-575 0007-4888 159:4<572 2015 159 10517 https://doi.org/10.1007/s10517-015-3016-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3016-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shabalkin I. N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Grigor'eva E. N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Gudkova M. N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Stukalov Yu N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/4(2015-08-01), 572-575 0007-4888 159:4<572 2015 159 10517