caa a22 4500 605536015 CHVBK 20210128100850.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10517-015-3015-5 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3015-5 Culturing of Mouse Mesenchymal Stem Cells on Poly-3-Hydroxybutyrate Scaffolds [Elektronische Daten] [N. Andreeva, A. Bonartsev, I. Zharkova, T. Makhina, V. Myshkina, E. Kharitonova, V. Voinova, G. Bonartseva, K. Shaitan, A. Belyavskii] We studied the possibility of long-term culturing of mouse mesenchymal stem cells on a porous scaffold made of biocompatible polymer poly-3-hydroxybutyrate. The cells remained viable for at least 2 months and passed more than 65 population doublings in culture. Culturing on the scaffold did not change surface phenotype of cells. 3D poly-3-hydroxybutyrate scaffolds are appropriate substrate for long-term culturing of mesenchymal stem cells. Springer Science+Business Media New York, 2015 mesenchymal stem cells nationallicence poly-3- hydroxybutyrate nationallicence polymer scaffold nationallicence Andreeva N. V. A. Engelhard Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia aut Bonartsev A. Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut Zharkova I. Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut Makhina T. Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia aut Myshkina V. Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia aut Kharitonova E. Physical Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut Voinova V. Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut Bonartseva G. Physical Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut Shaitan K. Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut Belyavskii A. V. A. Engelhard Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/4(2015-08-01), 567-571 0007-4888 159:4<567 2015 159 10517 https://doi.org/10.1007/s10517-015-3015-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3015-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Andreeva N. V. A. Engelhard Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Bonartsev A. Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 700 1- Zharkova I. Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 700 1- Makhina T. Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Myshkina V. Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Kharitonova E. Physical Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 700 1- Voinova V. Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 700 1- Bonartseva G. Physical Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 700 1- Shaitan K. Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 700 1- Belyavskii A. V. A. Engelhard Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/4(2015-08-01), 567-571 0007-4888 159:4<567 2015 159 10517