Evaluation of NAD(P)-Dependent Dehydrogenase Activities in Neutrophilic Granulocytes by the Bioluminescent Method
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| 024 | 7 | 0 | |a 10.1007/s10517-015-3049-8 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10517-015-3049-8 | ||
| 100 | 1 | |a Savchenko |D A. |u Research Institute of Medical Problems of the North, Siberian Division of the Russian Academy of Medical Sciences, Krasnoyarsk, Russia |4 aut | |
| 245 | 1 | 0 | |a Evaluation of NAD(P)-Dependent Dehydrogenase Activities in Neutrophilic Granulocytes by the Bioluminescent Method |h [Elektronische Daten] |c [A. Savchenko] |
| 520 | 3 | |a Bioluminescent method for measurements of the neutrophilic NAD(P)-dependent dehydrogenases (lactate dehydrogenase, NAD-dependent malate dehydrogenase, NADP-dependent decarboxylating malate dehydrogenase, NAD-dependent isocitrate dehydrogenase, and glucose- 6-phosphate dehydrogenase) is developed. The sensitivity of the method allows minimization of the volume of biological material for measurements to 104 neutrophils per analysis. The method is tried in patients with diffuse purulent peritonitis. Low levels of NADPH synthesis enzymes and high levels of enzymes determining the substrate flow by the Krebs cycle found in these patients can lead to attenuation of functional activity of cells. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a neutrophilic granulocytes |2 nationallicence | |
| 690 | 7 | |a metabolism |2 nationallicence | |
| 690 | 7 | |a enzyme activities |2 nationallicence | |
| 690 | 7 | |a dehydrogenases |2 nationallicence | |
| 690 | 7 | |a peritonitis |2 nationallicence | |
| 773 | 0 | |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 159/5(2015-09-01), 692-695 |x 0007-4888 |q 159:5<692 |1 2015 |2 159 |o 10517 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10517-015-3049-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10517-015-3049-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 100 |E 1- |a Savchenko |D A. |u Research Institute of Medical Problems of the North, Siberian Division of the Russian Academy of Medical Sciences, Krasnoyarsk, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 159/5(2015-09-01), 692-695 |x 0007-4888 |q 159:5<692 |1 2015 |2 159 |o 10517 | ||