caa a22 4500 605536201 CHVBK 20210128100851.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10517-015-3049-8 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3049-8 Savchenko A. Research Institute of Medical Problems of the North, Siberian Division of the Russian Academy of Medical Sciences, Krasnoyarsk, Russia aut Evaluation of NAD(P)-Dependent Dehydrogenase Activities in Neutrophilic Granulocytes by the Bioluminescent Method [Elektronische Daten] [A. Savchenko] Bioluminescent method for measurements of the neutrophilic NAD(P)-dependent dehydrogenases (lactate dehydrogenase, NAD-dependent malate dehydrogenase, NADP-dependent decarboxylating malate dehydrogenase, NAD-dependent isocitrate dehydrogenase, and glucose- 6-phosphate dehydrogenase) is developed. The sensitivity of the method allows minimization of the volume of biological material for measurements to 104 neutrophils per analysis. The method is tried in patients with diffuse purulent peritonitis. Low levels of NADPH synthesis enzymes and high levels of enzymes determining the substrate flow by the Krebs cycle found in these patients can lead to attenuation of functional activity of cells. Springer Science+Business Media New York, 2015 neutrophilic granulocytes nationallicence metabolism nationallicence enzyme activities nationallicence dehydrogenases nationallicence peritonitis nationallicence Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/5(2015-09-01), 692-695 0007-4888 159:5<692 2015 159 10517 https://doi.org/10.1007/s10517-015-3049-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3049-8 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Savchenko A. Research Institute of Medical Problems of the North, Siberian Division of the Russian Academy of Medical Sciences, Krasnoyarsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/5(2015-09-01), 692-695 0007-4888 159:5<692 2015 159 10517