caa a22 4500 605536260 CHVBK 20210128100851.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10517-015-3026-2 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3026-2 Role of Protein Kinase Akt Activation in Protective Effect of Ganglioside GM1 on PC12 Cells Exposed to H2O2 [Elektronische Daten] [I. Zakharova, T. Sokolova, A. Akhmetshina, N. Avrova] Micro- and nanomolar concentrations of ganglioside GM1 improved viability of neuronal PC12 cells under conditions of oxidative stress and reduced H2O2-induced ROS accumulation in these cells. These effects were more pronounced at micromolar concentrations. GM1 in concentrations of 100 nM and 10 μM significantly and substantially increased basal activity of protein kinase B (Akt) (the level of phosphorylated Akt form), but had virtually no effect on its expression in PC12 cells. In the presence of PI3K inhibitor LY294002 preventing protein kinase Akt activation, the protective effect of GM1 significantly decreased. These findings suggest that activation of protein kinase Akt by GM1 contributes to improvement of PC12 cell viability by this ganglioside. Springer Science+Business Media New York, 2015 ganglioside GM1 nationallicence protein kinase B (Akt) nationallicence PC12 cells nationallicence protective effect nationallicence formation of reactive oxygen species nationallicence Zakharova I. Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia aut Sokolova T. Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia aut Akhmetshina A. Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia aut Avrova N. Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/5(2015-09-01), 610-613 0007-4888 159:5<610 2015 159 10517 https://doi.org/10.1007/s10517-015-3026-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3026-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zakharova I. Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Sokolova T. Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Akhmetshina A. Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Avrova N. Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/5(2015-09-01), 610-613 0007-4888 159:5<610 2015 159 10517