caa a22 4500 605536309 CHVBK 20210128100851.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10517-015-3027-1 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3027-1 Expression of Glutamate and Glutamine Transporter Proteins in Neurovascular Unit Cells In Vitro [Elektronische Daten] [A. Morgun, N. Kuvacheva, E. Khilazheva, T. Taranushenko, A. Salmina] Glutamine transporter protein SLC1A5 and glutamate transporter protein EAAT2 responsible for cell-cell communication and energetic coupling were studied using in vitro model of multicellular neurovascular unit consisting of astrocytes, neurons, and endotheliocytes under standard conditions and during chemical hypoxia in vitro. Hypoxic damage to the neurovascular unit cells increased the number of SLC1A5-expressing cells and reduced the number of EAAT2-expressing astrocytes. Metabolic uncoupling in the neurovascular unit cells under hypoxic conditions resulted from abnormal expression of glutamine and glutamate transporter proteins, which is indicative of impaired glutamine and glutamate transport. Springer Science+Business Media New York, 2015 neurovascular unit nationallicence cell-cell interactions nationallicence transport proteins nationallicence hypoxia nationallicence Morgun A. V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia aut Kuvacheva N. V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia aut Khilazheva E. V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia aut Taranushenko T. V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia aut Salmina A. V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/5(2015-09-01), 614-616 0007-4888 159:5<614 2015 159 10517 https://doi.org/10.1007/s10517-015-3027-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3027-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morgun A. V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia aut NATIONALLICENCE 700 1- Kuvacheva N. V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia aut NATIONALLICENCE 700 1- Khilazheva E. V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia aut NATIONALLICENCE 700 1- Taranushenko T. V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia aut NATIONALLICENCE 700 1- Salmina A. V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/5(2015-09-01), 614-616 0007-4888 159:5<614 2015 159 10517