caa a22 4500 605536457 CHVBK 20210128100852.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10517-015-2932-7 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2932-7 Effects of Kagocel® on the Counts of Multipotent Stromal Cells, Expression of Cytokine Genes in Primary Cultures of Bone Marrow Stromal Cells, and Serum Cytokine Concentrations in CBA Mice [Elektronische Daten] [Yu. Gorskaya, V. Grabko, M. Konopleva, A. Suslov, V. Nesterenko] The efficiency of cloning of bone marrow multipotent stromal cells (ECF-MSC) from CBA mice and the MSC counts in the femoral bone increased 24 h after a single in vivo (but not in vitro) injection of kagocel (active substance of antiviral drug Kagocel ® ) 1.4 times (in response to 50-80 μg) and 4.6 times (in response to 250 μg). The maximum increase of ECF-MSC in response to 50 μg per mouse was detected just 1 h after Kagocel injection to intact mice and to mice previously receiving the drug for 3 days (2 and 1.7 times, respectively). The increase of ECF-MSC was 3-fold less intense in response to oral Kagocel in a dose of 250 μg/mouse vs. intraperitoneal Kagocel, ECF-MSC corresponding to its level in response to oral Poly (I:C). In vivo Kagocel led to emergence of proinflammatory cytokine IFN-γ, IL-1β, and IL-8 mRNA in primary cultures of bone marrow stromal cells. Serum concentrations of IL-2, IL-5, IL-10, GM-CSF, IFN-γ, TNF-α, IL-4, and IL-12 increased 1.5 and 2 times just 1 h after Kagocel injection in doses of 30-50 and 250 μg, respectively, to intact mice and to animals previously treated with the drug for 3 days. The cytokine concentrations normalized after 3 h and increased again after 24 h, though did not reach the levels recorded 1 h after the drug injection. These data indicated that the therapeutic and preventive effects of Kagocel, together with its previously demonstrated stimulation of α- and β-interferon production during several days, could be due to the capacity of this drug to increase the bone marrow ECF-MSC, serum cytokine concentrations, and induce the expression of proinflammatory cytokine genes in the bone marrow stromal cells 1 h after its injection. Springer Science+Business Media New York, 2015 multipotent stromal cells nationallicence Kagocel nationallicence Gorskaya Yu Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, and Laboratory of Immunity Mediators and Effectors, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Grabko V. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, and Laboratory of Immunity Mediators and Effectors, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Konopleva M. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, and Laboratory of Immunity Mediators and Effectors, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Suslov A. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, and Laboratory of Immunity Mediators and Effectors, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Nesterenko V. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, and Laboratory of Immunity Mediators and Effectors, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/2(2015-06-01), 240-244 0007-4888 159:2<240 2015 159 10517 https://doi.org/10.1007/s10517-015-2932-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2932-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gorskaya Yu Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, and Laboratory of Immunity Mediators and Effectors, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Grabko V. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, and Laboratory of Immunity Mediators and Effectors, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Konopleva M. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, and Laboratory of Immunity Mediators and Effectors, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Suslov A. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, and Laboratory of Immunity Mediators and Effectors, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Nesterenko V. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, and Laboratory of Immunity Mediators and Effectors, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/2(2015-06-01), 240-244 0007-4888 159:2<240 2015 159 10517