caa a22 4500 605536473 CHVBK 20210128100852.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10517-015-2931-8 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2931-8 Effects of Immunoregulatory Cytokines (IL-2, IL-7, and IL-15) on Expression of Gfi1 and U2afll4 Genes in T Cells at Different Stages of Differentiation [Elektronische Daten] [N. Sokhonevich, K. Yurova, A. Gutsol, O. Khaziakhmatova, I. Mazunin, L. Litvinova] Opposite effects of common γ-chain cytokines (IL-2, IL-7, and IL-15) on the expression of Gfi1 and U2af1l4 genes regulating alternative splicing of Ptprc gene in T cells at different stages of differentiation were demonstrated in vitro. Generally, produced a dose-dependent activating effect on T cells, while to the effects of rIL-7 and rIL-15 on T cells at different stages of differentiation were opposite to that of rIL-2: maximum concentrations of recombinant cytokines IL-7 and IL-15 produced the most pronounced inhibitory effect on U2af1l4 and to a lesser extent on Gfi1 gene expression, thus limiting activation of resting cells. This is consistent with their biological effects on T cells. In general, common γ-chain cytokines IL-2, IL-7, and IL-15 prevent differentiation of naïve T cells in vitro and limit activation of primed T cells in the absence of antigenic stimulus, which can contribute to the formation of cytokine imbalance. Springer Science+Business Media New York, 2015 cytokines nationallicence expression of Gfi1 and U2afll4 genes nationallicence memory T cells nationallicence naïve cells nationallicence alternative splicing nationallicence Sokhonevich N. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut Yurova K. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut Gutsol A. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut Khaziakhmatova O. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut Mazunin I. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut Litvinova L. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/2(2015-06-01), 236-239 0007-4888 159:2<236 2015 159 10517 https://doi.org/10.1007/s10517-015-2931-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2931-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sokhonevich N. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut NATIONALLICENCE 700 1- Yurova K. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut NATIONALLICENCE 700 1- Gutsol A. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut NATIONALLICENCE 700 1- Khaziakhmatova O. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut NATIONALLICENCE 700 1- Mazunin I. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut NATIONALLICENCE 700 1- Litvinova L. Laboratory of Immunology and Cell Technologies, Innovation Park, I. Kant Baltic Federal University, Kaliningrad, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/2(2015-06-01), 236-239 0007-4888 159:2<236 2015 159 10517