caa a22 4500 60553649X CHVBK 20210128100852.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10517-015-2925-6 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2925-6 Role of Purinergic Receptors of Erythrocytes in the Regulation of Conformation and Oxygen- and NO-Transporting Capacity of Hemoglobin [Elektronische Daten] [S. Kovalenko, A. Yusipovich, E. Parshina, G. Maksimov] Raman spectroscopy was used to investigate changes in hemoglobin conformation and its ability to transfer O2 and NO induced by activation of purinergic receptors of erythrocytes with extracellular ATP. It was found that addition of ATP in a final concentration of 0.1 mM and higher to erythrocyte suspension was followed by changes in hematoporphyrin conformation, while addition of ATP in a concentration of 1 mM and higher increased the proportion of oxyhemoglobin and NO-associated hemoglobin complexes. In case of purinergic receptors activation in blood erythrocytes, significant changes in hemoglobin conformation were observed only at a final ATP concentration of 5 mM, probably due to buffer properties of the blood. Springer Science+Business Media New York, 2015 ATP nationallicence hemoglobin nationallicence erythrocytes nationallicence Raman spectroscopy nationallicence purinergic receptors nationallicence Kovalenko S. M. V. Lomonosov Moscow State University, Moscow, Russia aut Yusipovich A. M. V. Lomonosov Moscow State University, Moscow, Russia aut Parshina E. M. V. Lomonosov Moscow State University, Moscow, Russia aut Maksimov G. M. V. Lomonosov Moscow State University, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/2(2015-06-01), 213-216 0007-4888 159:2<213 2015 159 10517 https://doi.org/10.1007/s10517-015-2925-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2925-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kovalenko S. M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 700 1- Yusipovich A. M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 700 1- Parshina E. M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 700 1- Maksimov G. M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/2(2015-06-01), 213-216 0007-4888 159:2<213 2015 159 10517