caa a22 4500 605536562 CHVBK 20210128100853.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10517-015-2937-2 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2937-2 Insulin Effects on Survival of Human Multiple Myeloma Cells [Elektronische Daten] [S. Shushanov, T. Kravtsova, Yu. Chernykh, N. Cheikina] Insulin effects of human multiple myeloma cell survival were studied on RPMI1640, RPMI8226, and IM9 lines differing by differentiation degree. The effects of exogenous insulin on tumor cell growth and survival varied. Insulin alone did not improve the viability of myeloma cells, while in combination with serum growth factors increased it. The IM9 cells with immunophenotype (CD138+, CD38-, CD45+, CD56-, CD19+) exhibited the highest sensitivity to serum growth factors, while RPMI1640 and RPMI8226 cells with (CD138+, CD38+, CD45-, CD56±, CD19-) immunophenotype were less sensitive. Studies of gene expression showed a significantly lower level of IRA mRNA expression in IM9 vs. RPMI1640 and RPMI8226 cells. Springer Science+Business Media New York, 2015 multiple myeloma nationallicence insulin nationallicence cell survival nationallicence mRNA expression nationallicence Shushanov S. Research Institute of Carcinogenesis, N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut Kravtsova T. Research Institute of Carcinogenesis, N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut Chernykh Yu Research Institute of Carcinogenesis, N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut Cheikina N. Research Institute of Carcinogenesis, N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/2(2015-06-01), 262-265 0007-4888 159:2<262 2015 159 10517 https://doi.org/10.1007/s10517-015-2937-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2937-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shushanov S. Research Institute of Carcinogenesis, N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Kravtsova T. Research Institute of Carcinogenesis, N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Chernykh Yu Research Institute of Carcinogenesis, N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Cheikina N. Research Institute of Carcinogenesis, N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/2(2015-06-01), 262-265 0007-4888 159:2<262 2015 159 10517