caa a22 4500 605536627 CHVBK 20210128100853.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10517-015-2944-3 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2944-3 Experimental Model to Study the Role of Retinoblastoma Gene Product (pRb) for Determination of Adipocyte Differentiation [Elektronische Daten] [B. Popov, P. Shilo, O. Zhidkova, A. Zaichik, N. Petrov] Using stable constitutive expression of retinoblastoma gene product (pRb) in polypotent mesenchymal 10T1/2 cells we obtained stable cell lines hyperexpressing functionally active or inactive mutant pRb. The cells producing active exogenous pRb demonstrated high sensitivity to adipocyte differentiation inductors, whereas production of inactive form of the exogenous protein suppressed adipocyte differentiation. The obtained lines can serve as the experimental model for studying the role of pRb in determination of adipocyte differentiation. Springer Science+Business Media New York, 2015 mesenchymal stem cells nationallicence adipocyte differentiation nationallicence pRb nationallicence Popov B. Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia aut Shilo P. Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia aut Zhidkova O. Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia aut Zaichik A. North-Western State Medical University, St. Petersburg, Russia aut Petrov N. Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/2(2015-06-01), 289-292 0007-4888 159:2<289 2015 159 10517 https://doi.org/10.1007/s10517-015-2944-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2944-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Popov B. Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Shilo P. Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Zhidkova O. Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Zaichik A. North-Western State Medical University, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Petrov N. Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 159/2(2015-06-01), 289-292 0007-4888 159:2<289 2015 159 10517