Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming
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| 024 | 7 | 0 | |a 10.1007/s10517-015-3112-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10517-015-3112-5 | ||
| 245 | 0 | 0 | |a Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming |h [Elektronische Daten] |c [A. Vdovin, A. Lupatov, I. Kholodenko, K. Yarygin] |
| 520 | 3 | |a Induced pluripotent cells were derived from adult human skin fibroblast by using two methods of reprogramming. Episomal transfection with vectors containing oriP/EBNA-1 sequence for delivery of reprogramming genes Oct4, Sox2, Klf4, L-Myc, and Lin28 proved to be more effective than viral transduction with Sendai virus-based vector: ~200 and 8 colonies per 105 cells were found on day 21 of culturing, respectively. Colonies of induced pluripotent cells obtained by these two methods expressed pluripotency marker Tra1-60. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a induced pluripotent stem cells |2 nationallicence | |
| 690 | 7 | |a transfection |2 nationallicence | |
| 690 | 7 | |a transduction |2 nationallicence | |
| 690 | 7 | |a reprogramming |2 nationallicence | |
| 690 | 7 | |a differentiation |2 nationallicence | |
| 700 | 1 | |a Vdovin |D A. |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia |4 aut | |
| 700 | 1 | |a Lupatov |D A. |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia |4 aut | |
| 700 | 1 | |a Kholodenko |D I. |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia |4 aut | |
| 700 | 1 | |a Yarygin |D K. |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia |4 aut | |
| 773 | 0 | |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 160/1(2015-11-01), 123-128 |x 0007-4888 |q 160:1<123 |1 2015 |2 160 |o 10517 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10517-015-3112-5 |q text/html |z Onlinezugriff via DOI |
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| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
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| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10517-015-3112-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vdovin |D A. |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lupatov |D A. |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kholodenko |D I. |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yarygin |D K. |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 160/1(2015-11-01), 123-128 |x 0007-4888 |q 160:1<123 |1 2015 |2 160 |o 10517 | ||