caa a22 4500 605536732 CHVBK 20210128100854.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10517-015-3112-5 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3112-5 Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming [Elektronische Daten] [A. Vdovin, A. Lupatov, I. Kholodenko, K. Yarygin] Induced pluripotent cells were derived from adult human skin fibroblast by using two methods of reprogramming. Episomal transfection with vectors containing oriP/EBNA-1 sequence for delivery of reprogramming genes Oct4, Sox2, Klf4, L-Myc, and Lin28 proved to be more effective than viral transduction with Sendai virus-based vector: ~200 and 8 colonies per 105 cells were found on day 21 of culturing, respectively. Colonies of induced pluripotent cells obtained by these two methods expressed pluripotency marker Tra1-60. Springer Science+Business Media New York, 2015 induced pluripotent stem cells nationallicence transfection nationallicence transduction nationallicence reprogramming nationallicence differentiation nationallicence Vdovin A. V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia aut Lupatov A. V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia aut Kholodenko I. V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia aut Yarygin K. V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 123-128 0007-4888 160:1<123 2015 160 10517 https://doi.org/10.1007/s10517-015-3112-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3112-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vdovin A. V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia aut NATIONALLICENCE 700 1- Lupatov A. V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia aut NATIONALLICENCE 700 1- Kholodenko I. V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia aut NATIONALLICENCE 700 1- Yarygin K. V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 123-128 0007-4888 160:1<123 2015 160 10517