caa a22 4500 605536740 CHVBK 20210128100854.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10517-015-3097-0 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3097-0 Effects of Liposomal Compositions with Oxidized Dextrans on Functional Activity of U937 Macrophage-Like Cells In Vitro [Elektronische Daten] [P. Kozhin, A. Chechushkov, N. Zaitseva, A. Lemza, E. Men'shchikova, A. Troitskii, V. Shkurupy] We studied the effects of liposomal pharmaceutical compositions with oxidized dextrans on functional activity of U937 monocyte/macrophage-like cells. Liposomes in the emulsion contained oxidized dextran with a molecular weights of 40 kDa or 70 kDa or isonicotinic acid hydrazide (INAH) conjugated with oxidized dextran (40 kDa). Cell viability was evaluated by MTT test; mitochondrial transmembrane potential and production of superoxide anion and H2O2 were studied by fluorescent methods. The studied compositions exhibited no cytotoxic effect and even improved cell viability and mitochondrial respiration. Liposomes with oxidized 40 kDa dextran, including those with INAH-conjugated dextran, inhibited production of superoxide anion, but increased H2O2 generation. Springer Science+Business Media New York, 2015 oxidized dextran nationallicence isonicotinic acid hydrazide nationallicence macrophages nationallicence viability nationallicence reactive oxygen species nationallicence Kozhin P. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut Chechushkov A. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut Zaitseva N. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut Lemza A. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut Men'shchikova E. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut Troitskii A. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut Shkurupy V. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 57-60 0007-4888 160:1<57 2015 160 10517 https://doi.org/10.1007/s10517-015-3097-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3097-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kozhin P. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Chechushkov A. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Zaitseva N. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Lemza A. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Men'shchikova E. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Troitskii A. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Shkurupy V. Research Institute of Experimental and Clinical Medicine, Novosibirsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 57-60 0007-4888 160:1<57 2015 160 10517