Production and Analysis of Biological Properties of Recombinant Human Apolipoprotein A-I
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| 024 | 7 | 0 | |a 10.1007/s10517-015-3113-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10517-015-3113-4 | ||
| 245 | 0 | 0 | |a Production and Analysis of Biological Properties of Recombinant Human Apolipoprotein A-I |h [Elektronische Daten] |c [A. Ryabchenko, M. Kotova, N. Tverdohleb, R. Knyazev, L. Polyakov] |
| 520 | 3 | |a Production of recombinant human apolipoprotein A-I (apoA-I) in E. coli cells is described and its biological properties are compared with those of natural protein. Recombinant apoA-I was isolated as a chimeric polypeptide and then processed to a mature form apoA-I (rapo-I). We studied the ability of the resulting protein to penetrate into hepatocyte nuclei and regulate the rate of DNA biosynthesis in complex with estriol. Penetration of rapoA-I conjugated with FITC into hepatocyte nuclei was demonstrated. rapoA-I-estriol and apoA-I-estriol complexes induced similar increase in DNA biosynthesis rate in isolated hepatocytes, which confi rms functional similarity of the obtained recombinant mature protein (rapoA-I) and native human apoA-I. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a recombinant apoA-I protein |2 nationallicence | |
| 690 | 7 | |a E. coli |2 nationallicence | |
| 690 | 7 | |a hepatocytes |2 nationallicence | |
| 690 | 7 | |a estriol |2 nationallicence | |
| 690 | 7 | |a DNA biosynthesis |2 nationallicence | |
| 700 | 1 | |a Ryabchenko |D A. |u Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia |4 aut | |
| 700 | 1 | |a Kotova |D M. |u Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia |4 aut | |
| 700 | 1 | |a Tverdohleb |D N. |u Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia |4 aut | |
| 700 | 1 | |a Knyazev |D R. |u Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia |4 aut | |
| 700 | 1 | |a Polyakov |D L. |u Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia |4 aut | |
| 773 | 0 | |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 160/1(2015-11-01), 129-133 |x 0007-4888 |q 160:1<129 |1 2015 |2 160 |o 10517 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10517-015-3113-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10517-015-3113-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ryabchenko |D A. |u Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kotova |D M. |u Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tverdohleb |D N. |u Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Knyazev |D R. |u Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Polyakov |D L. |u Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 160/1(2015-11-01), 129-133 |x 0007-4888 |q 160:1<129 |1 2015 |2 160 |o 10517 | ||