caa a22 4500 605536864 CHVBK 20210128100854.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10517-015-3113-4 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3113-4 Production and Analysis of Biological Properties of Recombinant Human Apolipoprotein A-I [Elektronische Daten] [A. Ryabchenko, M. Kotova, N. Tverdohleb, R. Knyazev, L. Polyakov] Production of recombinant human apolipoprotein A-I (apoA-I) in E. coli cells is described and its biological properties are compared with those of natural protein. Recombinant apoA-I was isolated as a chimeric polypeptide and then processed to a mature form apoA-I (rapo-I). We studied the ability of the resulting protein to penetrate into hepatocyte nuclei and regulate the rate of DNA biosynthesis in complex with estriol. Penetration of rapoA-I conjugated with FITC into hepatocyte nuclei was demonstrated. rapoA-I-estriol and apoA-I-estriol complexes induced similar increase in DNA biosynthesis rate in isolated hepatocytes, which confi rms functional similarity of the obtained recombinant mature protein (rapoA-I) and native human apoA-I. Springer Science+Business Media New York, 2015 recombinant apoA-I protein nationallicence E. coli nationallicence hepatocytes nationallicence estriol nationallicence DNA biosynthesis nationallicence Ryabchenko A. Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Kotova M. Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Tverdohleb N. Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Knyazev R. Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Polyakov L. Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 129-133 0007-4888 160:1<129 2015 160 10517 https://doi.org/10.1007/s10517-015-3113-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3113-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ryabchenko A. Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Kotova M. Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Tverdohleb N. Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Knyazev R. Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Polyakov L. Laboratory of Medical Biotechnology, Research Institute of Biochemistry, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 129-133 0007-4888 160:1<129 2015 160 10517