caa a22 4500 605536899 CHVBK 20210128100854.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10517-015-3090-7 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3090-7 Cellular Composition of the Spleen and Changes in Splenic Lysosomes in the Dynamics of Dyslipidemia in Mice Caused by Repeated Administration of Poloxamer 407 [Elektronische Daten] [N. Goncharova, A. Shurlygina, E. Mel'nikova, O. Karmatskikh, P. Avrorov, K. Loktev, T. Korolenko] We studied the effect of dyslipidemia induced by poloxamer 407 (300 mg/kg twice a week for 30 days) on cellular composition of the spleen and splenocyte lysosomes in mice. Changes in blood lipid profile included elevated concentrations of total cholesterol, aterogenic LDL, and triglycerides most pronounced in 24 h after the last poloxamer 407 injection; gradual normalization of lipid profile was observed in 4 days (except triglycerides) and 10 days. The most pronounced changes in the spleen (increase in organ weight and number of cells, inhibition in apoptosis, and reduced accumulation of vital dye acridine orange in lysosomes) were detected on day 4; on day 10, the indices returned to normal. Cathepsin D activity in the spleen also increased at these terms. The relationship between changes in the cellular composition of the spleen and dynamics of serum lipid profile in mice in dyslipidemia caused by repeated administrations of relatively low doses of poloxamer 407 is discussed. Springer Science+Business Media New York, 2015 dyslipidemia nationallicence poloxamer 407 nationallicence spleen nationallicence lysosomes nationallicence Goncharova N. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut Shurlygina A. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut Mel'nikova E. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut Karmatskikh O. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut Avrorov P. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut Loktev K. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut Korolenko T. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 28-31 0007-4888 160:1<28 2015 160 10517 https://doi.org/10.1007/s10517-015-3090-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3090-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Goncharova N. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Shurlygina A. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Mel'nikova E. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Karmatskikh O. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Avrorov P. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Loktev K. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Korolenko T. Research Institute of Physiology and Basic Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 28-31 0007-4888 160:1<28 2015 160 10517