caa a22 4500 605537011 CHVBK 20210128100855.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10517-015-3116-1 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3116-1 Optimized Protocol for Isolation of Multipotent Mesenchymal Stromal Cells from Human Umbilical Cord [Elektronische Daten] [Yu. Romanov, E. Balashova, N. Volgina, N. Kabaeva, T. Dugina, G. Sukhikh] Extraembryonic tissues, in particular, umbilical cord stroma are promising sources of multipotent mesenchymal stromal cells for regenerative medicine. In recent years, methods for isolation of mesenchymal stromal cells from different compartments of the umbilical cords based on enzymatic disaggregation of the tissue or on tissue explants have been proposed. Here we propose a protocol of isolation of multipotent mesenchymal stromal cells from the whole umbilical cord that combines the advantages of each approach and ensures sufficient cell yield for further experimental and clinical applications. A combination of short-term incubation of tissue fragments on cold collagenase solution followed by their culturing in the form of explants significantly increased the yield of cells with high proliferative activity, typical pluripotent mesenchymal stromal cell phenotype, and preserved differentiation capacity. Springer Science+Business Media New York, 2015 multipotent mesenchymal stromal cells nationallicence umbilical cord nationallicence explant nationallicence collagenase nationallicence Differentiation nationallicence Romanov Yu Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut Balashova E. Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut Volgina N. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut Kabaeva N. Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut Dugina T. CryoCenter Umbilical Cord Blood Bank, Moscow, Russia aut Sukhikh G. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 148-154 0007-4888 160:1<148 2015 160 10517 https://doi.org/10.1007/s10517-015-3116-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3116-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Romanov Yu Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Balashova E. Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Volgina N. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Kabaeva N. Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Dugina T. CryoCenter Umbilical Cord Blood Bank, Moscow, Russia aut NATIONALLICENCE 700 1- Sukhikh G. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 148-154 0007-4888 160:1<148 2015 160 10517