caa a22 4500 60553702X CHVBK 20210128100855.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10517-015-3115-2 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3115-2 Differentiation of Cholinergic Neurons in Rat Spinal Cord Under Conditions of Allotransplantation into a Peripheral Nerve and In Situ Development [Elektronische Daten] [E. Petrova, E. Kolos, D. Korzhevskii] The method of ectopic transplantation of embryonic anlages of CNS allows studying histoblastic potencies of progenitor cells developing under conditions of changed microenvironment. Some progenitor cells in the transplants of rat embryonic spinal cord retained their ability to express choline acetyltransferase after transplantation into the sciatic nerve of adult animals. Comparative analysis of cholinergic neurons in the neurotransplants and neurons formed in rat spinal cord during normal ontogeny showed that choline acetyltransferase-positive cells after transplantation into the nerve reached morphological differentiation of motor neurons at later terms than cells developing in situ. They were scattered one by one and did not form nuclear nerve centers. We did not fi nd structures similar to presynaptic cholinergic buds typical of intact spinal cord near these cells throughout the observation period. Solitary cholinergic neurons survived in the transplants for 19 months. Springer Science+Business Media New York, 2015 Nerve nationallicence embryonic spinal cord nationallicence cholinergic neurons nationallicence choline acetyltransferase nationallicence immunohistochemistry nationallicence Petrova E. Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology, Institute of Experimental Medicine, St. Petersburg, Russia aut Kolos E. Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology, Institute of Experimental Medicine, St. Petersburg, Russia aut Korzhevskii D. Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology, Institute of Experimental Medicine, St. Petersburg, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 141-147 0007-4888 160:1<141 2015 160 10517 https://doi.org/10.1007/s10517-015-3115-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3115-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Petrova E. Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology, Institute of Experimental Medicine, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Kolos E. Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology, Institute of Experimental Medicine, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Korzhevskii D. Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology, Institute of Experimental Medicine, St. Petersburg, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/1(2015-11-01), 141-147 0007-4888 160:1<141 2015 160 10517