caa a22 4500 605537119 CHVBK 20210128100855.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10517-015-3152-x doi (NATIONALLICENCE)springer-10.1007/s10517-015-3152-x Expression of B23/Nucleophosphamine Nonribosomal Nucleolar Protein in Smooth Muscle Tumors of the Corpus Uteri [Elektronische Daten] [A. Avdalyan, D. Kobyakov, V. Klimachev, I. Bobrov, A. Lazarev, A. Pichigina, E. Lushnikova, L. Nepomnyashchikh] Immunohistochemical study of B23/nucleophosmine (NPM), the key argyrophilic nonribosomal protein associated with the nucleolus-producing regions, in smooth muscle formations of the body of the uterus and myometrium was carried out. The uterus index, number of positively stained nucleoli in the nucleus, integral optical density, and area of positively stained nucleoli were studied by computer-aided image analysis. The results indicated significant differences in the integral optical density and less so in the areas of NPM+ nucleoli in leiomyosarcomas, but not in intact myometrium and leiomyomas. Coefficients of variations in the integral optical density and NPM area proved to be significant parameters for differentiating leiomyosarcomas by size (≤7 cm or larger), evaluating the malignancy grade and stage of the process. NPM parameters in leiomyomas and leiomyosarcomas differed significantly, depending on the levels of Ki-67 and p53 expression and the receptor status of leiomyosarcoma. The index of area and the integral optical density of NPM could serve a accessory objective differential diagnostic criteria in cellular and mitotically active liomyomas and well-differentiated leiomyosarcoma of the corpus uteri. Springer Science+Business Media New York, 2015 leiomyoma and leiomyosarcoma of the corpus uteri nationallicence nucleophosmine nationallicence immunohistochemistr nationallicence Avdalyan A. Altai Affiliated Department of N. N. Blokhin Russian Cancer Research Center, Barnaul, Russia aut Kobyakov D. Altai Affiliated Department of N. N. Blokhin Russian Cancer Research Center, Barnaul, Russia aut Klimachev V. Altai State Medical University, Barnaul, Russia aut Bobrov I. Altai State Medical University, Barnaul, Russia aut Lazarev A. Altai Affiliated Department of N. N. Blokhin Russian Cancer Research Center, Barnaul, Russia aut Pichigina A. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Lushnikova E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Nepomnyashchikh L. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/2(2015-12-01), 286-290 0007-4888 160:2<286 2015 160 10517 https://doi.org/10.1007/s10517-015-3152-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3152-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Avdalyan A. Altai Affiliated Department of N. N. Blokhin Russian Cancer Research Center, Barnaul, Russia aut NATIONALLICENCE 700 1- Kobyakov D. Altai Affiliated Department of N. N. Blokhin Russian Cancer Research Center, Barnaul, Russia aut NATIONALLICENCE 700 1- Klimachev V. Altai State Medical University, Barnaul, Russia aut NATIONALLICENCE 700 1- Bobrov I. Altai State Medical University, Barnaul, Russia aut NATIONALLICENCE 700 1- Lazarev A. Altai Affiliated Department of N. N. Blokhin Russian Cancer Research Center, Barnaul, Russia aut NATIONALLICENCE 700 1- Pichigina A. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Lushnikova E. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Nepomnyashchikh L. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/2(2015-12-01), 286-290 0007-4888 160:2<286 2015 160 10517