caa a22 4500 605537194 CHVBK 20210128100856.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10517-015-3124-1 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3124-1 Effect of Arachidonic Acid on the Rate of Oxygen Consumption in Isolated Cardiomyocytes from Intact Rats and Animals with Ischemic or Diabetic Injury to the Heart [Elektronische Daten] [M. Egorova, T. Kutsykova, S. Afanas'ev, S. Popov] We studied the rate of oxygen consumption by isolated cardiomyocytes from intact rats and animals with experimental myocardial infarction or streptozotocin-induced diabetes mellitus. The measurements were performed in standard incubation medium under various conditions of oxygenation and after addition of arachidonic acid (20 μmol/liter). Under normoxic conditions, arachidonic acid improves respiration of cardiomyocytes from intact animals, but reduces this parameter in cells isolated from animals with pathologies. The intensity of O2 consumption by cardiomyocytes from intact rats and animals with pathologies was shown to decrease during hypoxia. Addition of arachidonic acid aggravated inhibition of respiration for cardiomyocytes from intact rats and specimens with myocardial infarction, but had no effect in diabetes mellitus. The effect of arachidonic acid on oxygen consumption rate is probably mediated by a nonspecific mechanism realized at the mitochondrial level. Springer Science+Business Media New York, 2015 fatty acids nationallicence arachidonic acid nationallicence cardiomyocytes nationallicence diabetes nationallicence infarction nationallicence Egorova M. Research Institute of Cardiology, Tomsk, Russia aut Kutsykova T. Research Institute of Cardiology, Tomsk, Russia aut Afanas'ev S. Research Institute of Cardiology, Tomsk, Russia aut Popov S. Research Institute of Cardiology, Tomsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/2(2015-12-01), 190-192 0007-4888 160:2<190 2015 160 10517 https://doi.org/10.1007/s10517-015-3124-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3124-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Egorova M. Research Institute of Cardiology, Tomsk, Russia aut NATIONALLICENCE 700 1- Kutsykova T. Research Institute of Cardiology, Tomsk, Russia aut NATIONALLICENCE 700 1- Afanas'ev S. Research Institute of Cardiology, Tomsk, Russia aut NATIONALLICENCE 700 1- Popov S. Research Institute of Cardiology, Tomsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/2(2015-12-01), 190-192 0007-4888 160:2<190 2015 160 10517