caa a22 4500 605537216 CHVBK 20210128100856.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10517-015-3140-1 doi (NATIONALLICENCE)springer-10.1007/s10517-015-3140-1 Toxicity and Antiviral Activity of the Extracts of Submerged Mycelium of Nematophagous Duddingtonia flagrans Fungus in Vero Cell Culture [Elektronische Daten] [Zh. Ibragimova, G. Anan'ko, N. Kostina, T. Teplyakova, N. Mazurkova] We studied toxicity and antiviral activity of aqueous and ethanol extracts of bioactive substances from the biomass of nematophagous fungus Duddingtonia flagrans prepared by submerged culturing of the mycelium. It is found that both extracts were characterized by low toxicity for cultured Vero cells and inhibited reproduction of DNA-viruses in this cell line. Ethanol extract of the fungus exhibited higher in vitro antiviral activity against Herpes simplex virus type 2, ectromelia virus, and vaccinia virus than water extract, which can be due to higher content of proteins, polysaccharides, flavonols, catechins, or carotenes or more effective their combination. The extracts of cultured mycelium of Duddingtonia flagrans fungus containing a complex of bioactive substances can be used for creation of broad-spectrum antiviral drugs against DNA-viruses. Springer Science+Business Media New York, 2015 nematophagous fungus extract nationallicence orthopoxviruses nationallicence herpes simplex virus nationallicence Ibragimova Zh Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia aut Anan'ko G. Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia aut Kostina N. Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia aut Teplyakova T. Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia aut Mazurkova N. Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/2(2015-12-01), 246-248 0007-4888 160:2<246 2015 160 10517 https://doi.org/10.1007/s10517-015-3140-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3140-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ibragimova Zh Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia aut NATIONALLICENCE 700 1- Anan'ko G. Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia aut NATIONALLICENCE 700 1- Kostina N. Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia aut NATIONALLICENCE 700 1- Teplyakova T. Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia aut NATIONALLICENCE 700 1- Mazurkova N. Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk Region, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/2(2015-12-01), 246-248 0007-4888 160:2<246 2015 160 10517