caa a22 4500 605537275 CHVBK 20210128100856.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10517-015-3142-z doi (NATIONALLICENCE)springer-10.1007/s10517-015-3142-z Chemokine Receptors CXCR3 and CCR6 and Their Ligands in the Liver and Blood of Patients with Chronic Hepatitis C [Elektronische Daten] [N. Arsent'eva, A. Semenov, N. Lyubimova, Yu. Ostankov, D. Elezo, I. Kudryavtsev, V. Basina, E. Esaulenko, K. Kozlov, K. Zhdanov, A. Totolyan] We performed a comprehensive analysis of CCR6 and CXCR3 chemokine receptors and their ligands CCL20/MIP-3α, CXCL9/MIG, CXCL10/IP-10, and CXCL11/ITAC in the liver and blood of patients with chronic hepatitis C at different stages of the disease. TaqMan PCR was used to determine mRNA gene expression of chemokines and their receptors in liver specimens, xMAP multiplex analysis was performed to estimate the concentration of chemokines in blood plasma, and fl ow cytofluorometry was used to evaluate CCR6 and CXCR3 expression on peripheral blood lymphocyte populations. In the liver of patients with hepatitis C, mRNA expression of CXCL10, CCR6, and CXCR3 genes increases with fibrosis progression in the liver tissue. In the plasma, concentrations of all studied chemokines increased depending on the stage of liver fibrosis, CCR6 and CXCR3 expression was changed in various lymphocyte populations. Thus, chemokines are involved in the immunopathogenesis and fibrogenesis in chronic viral hepatitis C. The results suggest using these chemokines in the diagnosis and prognosis of the disease. Springer Science+Business Media New York, 2015 viral hepatitis C nationallicence chemokines nationallicence fibrogenesis nationallicence immunopathogenesis nationallicence biomarkers nationallicence Arsent'eva N. Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut Semenov A. Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut Lyubimova N. Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut Ostankov Yu Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut Elezo D. Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut Kudryavtsev I. Institute of Experimental Medicine, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut Basina V. St. Petersburg State Pediatric Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia aut Esaulenko E. St. Petersburg State Pediatric Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia aut Kozlov K. S. M. Kirov Military Medical Academy, St. Petersburg, Russia aut Zhdanov K. S. M. Kirov Military Medical Academy, St. Petersburg, Russia aut Totolyan A. Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/2(2015-12-01), 252-255 0007-4888 160:2<252 2015 160 10517 https://doi.org/10.1007/s10517-015-3142-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-3142-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arsent'eva N. Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Semenov A. Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Lyubimova N. Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Ostankov Yu Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Elezo D. Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Kudryavtsev I. Institute of Experimental Medicine, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Basina V. St. Petersburg State Pediatric Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Esaulenko E. St. Petersburg State Pediatric Medical University, Ministry of Health of the Russian Federation, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Kozlov K. S. M. Kirov Military Medical Academy, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Zhdanov K. S. M. Kirov Military Medical Academy, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Totolyan A. Pasteur St. Petersburg Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 160/2(2015-12-01), 252-255 0007-4888 160:2<252 2015 160 10517