caa a22 4500 605537925 CHVBK 20210128100859.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10517-015-2763-6 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2763-6 Effects of Low-Doses of Bacillus Spp [Elektronische Daten] from Permafrost on Differentiation of Bone Marrow Cells [L. Kalyonova, M. Novikova, E. Kostolomova] The effects of a new microorganism species (Bacillus spp., strain M3) isolated from permafrost specimens from Central Yakutia (Mamontova Mountain) on the bone marrow hemopoiesis were studied on laboratory mice. Analysis of the count and immunophenotype of bone marrow cells indicated that even in low doses (1000-5000 microbial cells) these microorganisms modulated hemopoiesis and lymphopoiesis activity. The percentage of early hemopoietic precursors (CD117+CD34-) increased, intensity of lymphocyte precursor proliferation and differentiation (CD25+CD44-) decreased, and the percentage of lymphocytes released from the bone marrow (CD25+CD44+) increased on day 21 after injection of the bacteria. These changes in activity of hemopoiesis were associated with changes in the level of regulatory T lymphocytes (reduced expression of TCRαβ) and were most likely compensatory. The possibility of modulating hemopoiesis activity in the bone marrow by low doses of one microorganism strain isolated from the permafrost could be useful for evaluating the effects of other low dose bacteria on the bone marrow hemopoiesis. Springer Science+Business Media New York, 2015 permafrost microorganisms nationallicence bone marrow cell differentiation nationallicence Kalyonova L. Tyumen Research Center, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia aut Novikova M. Tyumen Research Center, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia aut Kostolomova E. Tyumen Research Center, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/3(2015-01-01), 364-367 0007-4888 158:3<364 2015 158 10517 https://doi.org/10.1007/s10517-015-2763-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2763-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kalyonova L. Tyumen Research Center, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Novikova M. Tyumen Research Center, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Kostolomova E. Tyumen Research Center, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/3(2015-01-01), 364-367 0007-4888 158:3<364 2015 158 10517