caa a22 4500 605538050 CHVBK 20210128100900.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10517-015-2805-0 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2805-0 Role of Peptide Bond in the Realization of Biological Activity of Short Peptides [Elektronische Daten] [V. Khavinson, S. Tarnovskaya, N. Lin'kova, N. Chervyakova, T. Nichik, E. Elashkina, N. Chalisova] We performed a comparative analysis of biological activity of Lys-Glu peptide and its amino acid constituents. It was established that Lys-Glu stimulated proliferation of splenic cells in organotypic culture, while the mixture of glutamic acid and lysine inhibited culture growth. Using the method of molecular docking, we showed that glutamic acid, lysine, and Lys-Glu peptide can interact with different DNA sequences. The energy of interaction and the most beneficial localization of glutamic acid, lysine, and Lys-Glu peptide in DNA molecule was calculated. We demonstrated the interaction of the peptide and amino acids with DNA along the minor groove. The energy of DNA interaction with the peptide is higher than with individual amino acids. The peptide bonds increase the interaction of Lys-Glu peptide with DNA, which potentiates the biological effect on cell proliferation in organotypic culture of splenic cells. Springer Science+Business Media New York, 2015 dipeptide nationallicence amino acids nationallicence molecular modeling nationallicence docking nationallicence organotypic culture nationallicence Khavinson V. I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia aut Tarnovskaya S. St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia aut Lin'kova N. St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia aut Chervyakova N. St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia aut Nichik T. St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia aut Elashkina E. St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia aut Chalisova N. I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 551-554 0007-4888 158:4<551 2015 158 10517 https://doi.org/10.1007/s10517-015-2805-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2805-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khavinson V. I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Tarnovskaya S. St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Lin'kova N. St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Chervyakova N. St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Nichik T. St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Elashkina E. St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Chalisova N. I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 551-554 0007-4888 158:4<551 2015 158 10517