caa a22 4500 605538069 CHVBK 20210128100900.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10517-015-2804-1 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2804-1 Expression of Ryanodine Receptor mRNA and Sarcoplasmic Ca2+-ATPase mRNA in the Myocardium and Intracellular Reorganization of Cardiomyocytes in Dyslipidemia and during Verapamil Treatment [Elektronische Daten] [E. Yuzhik, E. Lushnikova, M. Klinnikova, V. Pichigin, L. Nepomnyashchikh] In experiments on rats, atherogenic diet led to hypercholesterolemia, while addition of verapamil to the diet led to the development of hypertriglyceridemia in these animals. Dyslipidemia induced signifi cant changes in the cardiomyocyte ultrastructure (lytic changes in myofi brils and sarcoplasmic matrix and aggravation of autophagocytosis) that were most pronounced after addition of mercazolyl alone to the diet. After 30-day atherogenic diet, we observed a decrease in the relative content of RyR2 mRNA (by 67-73%, p<0.01) and SERCA2a mRNA (by 75-91%, p<0.01) in the myocardium. In 64 days these parameters remained reduced: by 64-72% (p<0.05) and 54-62% (p<0.05), respectively. Verapamil treatment reduced the severity and number of lytic lesions in cardiomyocytes, induced considerable glycogen accumulation in the sarcoplasm and its sequestration, promoted normalization, and prevented pronounced decrease of the relative RyR2 mRNA and SERCA2a mRNA in rat myocardium. Springer Science+Business Media New York, 2015 dyslipidemia nationallicence cardiomyocyte ultrastructure nationallicence verapamil nationallicence Ca 2+ transport nationallicence electron microscopy nationallicence Yuzhik E. Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Lushnikova E. Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Klinnikova M. Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Pichigin V. Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Nepomnyashchikh L. Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 544-550 0007-4888 158:4<544 2015 158 10517 https://doi.org/10.1007/s10517-015-2804-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2804-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yuzhik E. Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Lushnikova E. Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Klinnikova M. Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Pichigin V. Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Nepomnyashchikh L. Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 544-550 0007-4888 158:4<544 2015 158 10517