Modeling and Integral X-Ray, Optical, and MRI Visualization of Multiorgan Metastases of Orthotopic 4T1 Breast Carcinoma in BALB/c Mice
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| 024 | 7 | 0 | |a 10.1007/s10517-015-2810-3 |2 doi |
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| 245 | 0 | 0 | |a Modeling and Integral X-Ray, Optical, and MRI Visualization of Multiorgan Metastases of Orthotopic 4T1 Breast Carcinoma in BALB/c Mice |h [Elektronische Daten] |c [V. Baklaushev, N. Grinenko, G. Yusubalieva, M. Abakumov, I. Gubskii, S. Cherepanov, I. Kashparov, M. Burenkov, E. Rabinovich, N. Ivanova, O. Antonova, V. Chekhonin] |
| 520 | 3 | |a A model of highly metastasizing orthotopic allogeneic breast carcinoma was reproduced and standardized in experiments on BALB/c mice. 4T1 cells characterized by high metastatic activity were transfected with red fluorescent protein (RFP) gene or firefly luciferase (Luc2) gene. Unmodified 4T1 cells and modified 4T1-RFP and 4T1-Luc2 cells were subcutaneously injected to mature female mice into the second mammary fat pads. Quantitative evaluation of the primary node and visceral metastases was performed using magnetic-resonance imaging, X-ray and optical tomography. Modification of 4T1 cells with RFP gene considerably reduced their invasive and metastatic potential and led to spontaneous regression of the primary tumor in 20% cases. Modification of 4T1 cells with Luc2 gene had practically no effect on proliferative, invasive, and metastatic characteristics of the tumor and provided the possibility of quantitative analysis of the primary tumor dynamics by the luminescence intensity. The survival median in mice receiving unmodified 4T1 cells and transfected 4T1-RFP and 4Т1-Luc2 cells was 32, 42, and 38 days, respectively. Neither primary node nor tumor metastases accumulated gadolinium-containing contrast agent and Alasens fluorescent tracer. After implantation of 4T1 and 4Т1-Luc2 cells, multiple metastases were more often detected in the lungs, liver, spleen, spine, and regional lymph nodes and less frequently in the brain, which corresponded to metastasizing profile of human breast cancer. The developed model of orthotopic breast carcinoma 4T1 in BALB/c mice with complex detection of multiple organ metastases using X-ray microCT, optical, and MRI can be recommended for preclinical studies of new antitumor preparations. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a 4T1 breast carcinoma |2 nationallicence | |
| 690 | 7 | |a multiple organ metastases |2 nationallicence | |
| 690 | 7 | |a optical tomography |2 nationallicence | |
| 690 | 7 | |a microCT |2 nationallicence | |
| 690 | 7 | |a magnetic-resonance imaging |2 nationallicence | |
| 700 | 1 | |a Baklaushev |D V. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | |
| 700 | 1 | |a Grinenko |D N. |u Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | |
| 700 | 1 | |a Yusubalieva |D G. |u Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | |
| 700 | 1 | |a Abakumov |D M. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | |
| 700 | 1 | |a Gubskii |D I. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | |
| 700 | 1 | |a Cherepanov |D S. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | |
| 700 | 1 | |a Kashparov |D I. |u Institute of Protein Research, Russian Academy of Sciences, Moscow, Russia |4 aut | |
| 700 | 1 | |a Burenkov |D M. |u Farm-Sintez Company, Moscow, Russia |4 aut | |
| 700 | 1 | |a Rabinovich |D E. |u Farm-Sintez Company, Moscow, Russia |4 aut | |
| 700 | 1 | |a Ivanova |D N. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | |
| 700 | 1 | |a Antonova |D O. |u Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | |
| 700 | 1 | |a Chekhonin |D V. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | |
| 773 | 0 | |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 158/4(2015-02-01), 581-588 |x 0007-4888 |q 158:4<581 |1 2015 |2 158 |o 10517 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10517-015-2810-3 |q text/html |z Onlinezugriff via DOI |
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| 908 | |D 1 |a research-article |2 jats | ||
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| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10517-015-2810-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Baklaushev |D V. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Grinenko |D N. |u Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yusubalieva |D G. |u Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Abakumov |D M. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gubskii |D I. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cherepanov |D S. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kashparov |D I. |u Institute of Protein Research, Russian Academy of Sciences, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Burenkov |D M. |u Farm-Sintez Company, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rabinovich |D E. |u Farm-Sintez Company, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ivanova |D N. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Antonova |D O. |u Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Ministry of Health of the Russian Federation, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chekhonin |D V. |u Research and Education Center Medical Nanobiotechnologies, Russian State Medical University, Moscow, Russia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Bulletin of Experimental Biology and Medicine |d Springer US; http://www.springer-ny.com |g 158/4(2015-02-01), 581-588 |x 0007-4888 |q 158:4<581 |1 2015 |2 158 |o 10517 | ||