caa a22 4500 60553814X CHVBK 20210128100900.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10517-015-2781-4 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2781-4 Analysis of Toxicity Biomarkers of Fullerene C60 Nanoparticles by Confocal Fluorescent Microscopy [Elektronische Daten] [V. Shipelin, T. Smirnova, I. Gmoshinskii, V. Tutelyan] The methods of laser confocal microscopy were employed to study the changes in rat target organs (iliac mucosa and liver) provoked by peroral administration of dispersion of nanosized (31 nm) multimolecular fullerene C60 particles in doses of 0.1, 1.0, and 10 mg/kg body weight over 92 days. The micropreparations were selectively stained with fluorescent dyes to mark the cell nuclei (DAPI), actin microfilaments (fluorescently labeled phalloidin), and the membrane proteins CD106, CD31, and claudins in tight junctions (fluorescently labeled monoclonal antibodies). In rats treated with fullerene in the examined doses, the iliac mucosa demonstrated normal morphology of the villi. There were no signs of inflammation and no alterations in the actin fi laments of cytoskeleton and in enterocytic tight junctions. The count of CD106+ and CD31+ cells did not change. The highest examined doses of fullerene (1 and 10 mg/kg body weight) increased population and modified distribution of hepatic CD106+ cells. They also resulted in accumulation of cytoplasmic granules presumably identified as Kupffer macrophages without any signs of visible inflammation or necrotic areas. This phenomenon can reflect the early stages of toxic reaction being a sensitive bioindicator of the damage produced by administered fullerene C60 in the hepatic tissue. Springer Science+Business Media New York, 2015 fullerene nationallicence toxicity nationallicence ileum nationallicence liver nationallicence confocal microscopy nationallicence Shipelin V. State Research Institute of Nutrition, Moscow, Russia aut Smirnova T. State Research Institute of Nutrition, Moscow, Russia aut Gmoshinskii I. State Research Institute of Nutrition, Moscow, Russia aut Tutelyan V. State Research Institute of Nutrition, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 443-449 0007-4888 158:4<443 2015 158 10517 https://doi.org/10.1007/s10517-015-2781-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2781-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shipelin V. State Research Institute of Nutrition, Moscow, Russia aut NATIONALLICENCE 700 1- Smirnova T. State Research Institute of Nutrition, Moscow, Russia aut NATIONALLICENCE 700 1- Gmoshinskii I. State Research Institute of Nutrition, Moscow, Russia aut NATIONALLICENCE 700 1- Tutelyan V. State Research Institute of Nutrition, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 443-449 0007-4888 158:4<443 2015 158 10517