caa a22 4500 605538182 CHVBK 20210128100900.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10517-015-2794-z doi (NATIONALLICENCE)springer-10.1007/s10517-015-2794-z In Vitro Effects of Nanosized Diamond Particles on Macrophages [Elektronische Daten] [V. Shkurupy, S. Arkhipov, D. Neshchadim, E. Akhramenko, A. Troitskii] The effects of synthetic diamond nanoparticles (4-6 nm) on mouse macrophage biotropism and biocompatibility and the modulation of the macrophage functions (expression of IL-1α, TNF-α, GM-CSF, bFGF, and TGF-β) by nanoparticles in different concentrations were studied in vitro during exposure of different duration. Macrophage endocytosis of nanodiamonds increased with increasing the concentration of nanoparticles in culture and incubation time. Nanodiamonds exhibited high biotropism and biocompatibility towards macrophages; in doses of 10-20 μg/ml, they induced expression of GM-CSF and TGF-β, inhibited expression of bFGF, and did not stimulate IL-1α and TNF-α. These data indicate that nanodiamond capture by macrophages in the studied experimental model led to modulation of the functional status of macrophages that determine their capacity to stimulate reparative processes without increasing proinflammatory and profibrogenic status. Springer Science+Business Media New York, 2015 nanodiamonds nationallicence macrophages nationallicence biocompatibility nationallicence cytokine expression nationallicence in vitro nationallicence Shkurupy V. Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Arkhipov S. Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Neshchadim D. Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Akhramenko E. Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Troitskii A. Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 500-503 0007-4888 158:4<500 2015 158 10517 https://doi.org/10.1007/s10517-015-2794-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2794-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shkurupy V. Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Arkhipov S. Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Neshchadim D. Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Akhramenko E. Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Troitskii A. Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 500-503 0007-4888 158:4<500 2015 158 10517