caa a22 4500 605538220 CHVBK 20210128100901.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10517-015-2786-z doi (NATIONALLICENCE)springer-10.1007/s10517-015-2786-z Effects of Combined Treatment with Complex S. typhimurium Antigens and Factors Stimulating Osteogenesis (Curettage, BMP-2) on Multipotent Bone Marrow Stromal Cells and Serum Concentration of Cytokines in CBA Mice [Elektronische Daten] [Yu. Gorskaya, T. Danilova, A. Karyagina, V. Lunin, V. Grabko, M. Bartov, A. Gromov, T. Grunina, L. Soboleva, I. Shapoval, V. Nesterenko] The content of multipotent stromal cells (MSC) in the bone marrow and efficiency of their cloning (ECF-MSC) increased by 3 times 1 day after administration of complex S. typhimurium antigens to CBA mice, while the relative content of alkaline phosphatase-positive MSC colonies (marker of osteogenesis; P+ colonies) decreased from 14% (control) to 3%. After administration of the complex S. typhimurium antigens to CBA mice 3 h after (or 3 h before) curettage or treatment with morphogenetic protein (BMP-2), the content of MSC and ECF-MSC decreased on the next day by ~3 times in comparison with animals receiving antigens alone and approached the control level. The relative content of P+ colonies increased to 20 and 35%, respectively, in comparison with animals receiving antigens (3%), but was signifi cantly lower than after curettage (34%) or BMP-2 (42%) administration. Expression of IL-1β, IL-6, IL-12, TNF-α, and IFN-γ genes in the primary cultures of stromal bone marrow cells induced by antigen administration was suppressed, while the concentrations of IL-12 and TNF-α in the culture medium sharply decreased after antigen treatment in combination with curettage or BMP-2 administration. Administration of complex S. typhimurium antigens after pretreatment with BMP-2 (3 h before) was associated with a decrease in serum levels of IL-2, IFN-γ, IL-12, and TNF-α in mice receiving BMP-2+S. typhimurium group 4 h after treatment in comparison with the animals receiving only S. typhimurium antigens alone by 1.9, 4.4, 1.5, and 6 times, respectively, i.e. to normal level or below it, while the concentration of IL-10 increased by almost 2 times, which probably reflected anti-inflammatory properties of BMP-2. These data probably attest to competitive relations between osteogenesis and immune response at the level of MSC. Springer Science+Business Media New York, 2015 stromal cells nationallicence immune response nationallicence osteogenic properties nationallicence Gorskaya Yu Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Danilova T. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Karyagina A. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Lunin V. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Grabko V. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Bartov M. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Gromov A. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Grunina T. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Soboleva L. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Shapoval I. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Nesterenko V. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 465-470 0007-4888 158:4<465 2015 158 10517 https://doi.org/10.1007/s10517-015-2786-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2786-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gorskaya Yu Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Danilova T. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Karyagina A. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Lunin V. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Grabko V. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Bartov M. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Gromov A. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Grunina T. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Soboleva L. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Shapoval I. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 700 1- Nesterenko V. Laboratory of Immunity Regulation, Laboratory of Bioactive Nanostructures, Laboratory of Microbiology of Latent Infections, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, the Ministry of Health of the Russian Federation, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 465-470 0007-4888 158:4<465 2015 158 10517