caa a22 4500 605538247 CHVBK 20210128100901.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10517-015-2809-9 doi (NATIONALLICENCE)springer-10.1007/s10517-015-2809-9 Effect of Proinflammatory Activation on F-Actin Distribution in Cultured Human Endothelial Cells under Conditions of Experimental Microgravity [Elektronische Daten] [E. Rudimov, S. Buravkov, E. Andreeva, L. Buravkova] We compared the state of actin cytoskeleton, morphology, and expression of VE-cadherin in endothelial cells of human umbilical cord vein under conditions of TNF-α-mediated activation and microgravity modeling and found that 3D-clinorotation for 24 h impaired the integrity of endothelial monolayer, altered cell morphology, induced cytoskeleton reorganization, and reduced the expression of VE-cadherin. The combination of experimental microgravity and proinflammatory activation led to more pronounced clearing of the perinuclear space from microfilaments and accumulation of depolymerized actin, which confi rms additive effect of the studied factors on actin cytoskeleton of endothelial cells. Springer Science+Business Media New York, 2015 endothelial cells nationallicence microgravity nationallicence proinfl ammatory activation nationallicence cytoskeleton nationallicence Rudimov E. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia aut Buravkov S. M. V. Lomonosov Moscow State University, Moscow, Russia aut Andreeva E. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia aut Buravkova L. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia aut Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 573-580 0007-4888 158:4<573 2015 158 10517 https://doi.org/10.1007/s10517-015-2809-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10517-015-2809-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rudimov E. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Buravkov S. M. V. Lomonosov Moscow State University, Moscow, Russia aut NATIONALLICENCE 700 1- Andreeva E. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Buravkova L. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 773 0- Bulletin of Experimental Biology and Medicine Springer US; http://www.springer-ny.com 158/4(2015-02-01), 573-580 0007-4888 158:4<573 2015 158 10517