Comparative Analysis of the Expression of Surface Markers on Fibroblasts and Fibroblast-Like Cells Isolated from Different Human Tissues

Verfasser / Beitragende:
[A. Lupatov, A. Vdovin, I. Vakhrushev, R. Poltavtseva, K. Yarygin]
Ort, Verlag, Jahr:
2015
Enthalten in:
Bulletin of Experimental Biology and Medicine, 158/4(2015-02-01), 537-543
Format:
Artikel (online)
ID: 605538352
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024 7 0 |a 10.1007/s10517-015-2803-2  |2 doi 
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245 0 0 |a Comparative Analysis of the Expression of Surface Markers on Fibroblasts and Fibroblast-Like Cells Isolated from Different Human Tissues  |h [Elektronische Daten]  |c [A. Lupatov, A. Vdovin, I. Vakhrushev, R. Poltavtseva, K. Yarygin] 
520 3 |a Expression of 20 surface markers was analyzed in cultures of mesenchymal stromal cells of the umbilical cord, fibroblasts from adult and fetal human skin, and fi broblast-like cells of fetal liver was analyzed by fl ow cytometry. The studied cultures did not express hemopoietic cells markers, but were positive for CD73, CD90, and CD105 markers recommended by the International Society of Cell Therapy for the identifi cation of the multipotent mesenchymal stromal cells. Fetal liver fi broblast-like cells were positive for CD54; this marker was absent in skin fi broblast cultures, but was expressed by umbilical cord mesenchymal stromal cells. Further study of these cells revealed a minor subpopulation of cells co-expressing CD24 and CD90 or CD24 and CD54. We hypothesized that these cells probably participate in epithelial mesenchymal transition. 
540 |a Springer Science+Business Media New York, 2015 
690 7 |a multipotent mesenchymal stromal cells  |2 nationallicence 
690 7 |a umbilical cord  |2 nationallicence 
690 7 |a fibroblasts  |2 nationallicence 
690 7 |a fetal liver  |2 nationallicence 
690 7 |a surface markers  |2 nationallicence 
700 1 |a Lupatov  |D A.  |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia  |4 aut 
700 1 |a Vdovin  |D A.  |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia  |4 aut 
700 1 |a Vakhrushev  |D I.  |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia  |4 aut 
700 1 |a Poltavtseva  |D R.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
700 1 |a Yarygin  |D K.  |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia  |4 aut 
773 0 |t Bulletin of Experimental Biology and Medicine  |d Springer US; http://www.springer-ny.com  |g 158/4(2015-02-01), 537-543  |x 0007-4888  |q 158:4<537  |1 2015  |2 158  |o 10517 
856 4 0 |u https://doi.org/10.1007/s10517-015-2803-2  |q text/html  |z Onlinezugriff via DOI 
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900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
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950 |B NATIONALLICENCE  |P 700  |E 1-  |a Lupatov  |D A.  |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Vdovin  |D A.  |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Vakhrushev  |D I.  |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Poltavtseva  |D R.  |u V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Yarygin  |D K.  |u V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Bulletin of Experimental Biology and Medicine  |d Springer US; http://www.springer-ny.com  |g 158/4(2015-02-01), 537-543  |x 0007-4888  |q 158:4<537  |1 2015  |2 158  |o 10517